Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11356

R&D Systems, part of Bio-Techne
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AVI11356-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P04626.1

Conjugate

Biotin

Applications

Bioactivity

View all ErbB2/Her2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ErbB2/Her2 protein
Human ErbB2
(Thr23-Thr652)
Accession # P04626.1		 Avi-tag 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr23

Predicted Molecular Mass

73 kDa

SDS-PAGE

92-112 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody (Catalog # MAB9589) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ErbB2/Her2 Avi-tag His-tag (Catalog # AVI11356) binds with an ED50 of 0.500-6.00 ng/mL.

Scientific Data Images for Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein, CF

Biotinylated Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein Binding Activity.

When Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody (MAB9589) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein (Catalog # AVI11356) binds with an ED50 of 0.500-6.00 ng/mL.

Biotinylated Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein (Catalog # AVI11356) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 92-112 kDa.

Formulation, Preparation and Storage

AVI11356
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I transmembrane glycoprotein member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factors. It is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among the ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Human ErbB2 consists of 1255 amino acids (aa) including a 22 aa signal sequence, a 630 aa extracellular domain (ECD), a 23 aa transmembrane region, and a 580 aa cytoplasmic domain.  Within the ECD, human ErbB2 shares and 86% aa sequence identity with mouse and rat ErbB2.  Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10). Our Avi-tag Biotinylated human ErbB2/Her2 His-tag protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Coussens, L. et al. (1985) Science 230:1132.
  2. Yamamoto, T. et al. (1986) Nature 319:230.
  3. Kanai, Y. et al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Gene Symbol

ERBB2

UniProt

P04626.1

Additional ErbB2/Her2 Products

Product Documents for Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein, CF

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Product Specific Notices for Recombinant Human ErbB2/Her2 Avi-tag His-tag Protein, CF

For research use only

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