Recombinant Human Erythropoietin R Protein
R&D Systems, part of Bio-Techne | Catalog # 307-ER
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Erythropoietin R protein
Ala25-Pro250
Ala25-Pro250
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala25
Predicted Molecular Mass
25 kDa
SDS-PAGE
26-34 kDa, reducing conditions
Activity
Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 60-300 ng/mL in the presence of 0.2 U/mL of rhEpo.
The ED50 for this effect is 60-300 ng/mL in the presence of 0.2 U/mL of rhEpo.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 307-ER
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose and with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 307-ER/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Erythropoietin R
Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the erythropoietin receptor (Epo R). The genes for human and mouse Epo R have been cloned and characterized. The full-length human Epo R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) residues (a 24 aa residue hydrophobic signal sequence, a 226 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 236 aa residue cytoplasmic domain). At the protein sequence level, the human Epo R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding a truncated form of the Epo R as well as the soluble form of Epo R has been found. The presence of a soluble form of the Epo R has also been detected on human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.
References
- Barber, D.L. and A.D. D’Andrea (1992) Seminars in Hematology 29:293.
- Youssoufian, H. et al. (1993) Blood 9:2223.
- Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93 - 104.
- Baynes, R.D. et al. (1993) Blood 82:2088.
Long Name
Erythropoietin Receptor
Alternate Names
EpoR
Gene Symbol
EPOR
UniProt
Additional Erythropoietin R Products
Product Documents for Recombinant Human Erythropoietin R Protein
Product Specific Notices for Recombinant Human Erythropoietin R Protein
For research use only
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