Chinese Hamster Ovary cell line, CHO-derived human Exostosin 1/2 Heterodimer protein
Arg29-Leu746 with a C-terminal 2-His and HA tag (EXT1) & Ser53-Leu718 with a C-terminal 6-His tag (EXT2)

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg29 (EXT1) & Ser53 (EXT2)

Predicted Molecular Mass

84 kDa (EXT1) & 77 kDa (EXT2)

SDS-PAGE

69-85 kDa, reducing conditions

Activity

Measured by its ability to transfer GlcNAc and GlcA from donor substrates to heparan sulfate.
The specific activity is >225 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8567-GT

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Exostosin 1/2 Heterodimer

Exostosin (EXT) family of glycosyltransferases are involved in heparan sulfate (HS) and heparin biosynthesis (1). Mutations of these enzymes are the causes of hereditary multiple exostoses, which are characterized by formation of numerous cartilage-capped, benign bone tumors (osteocartilaginous exostoses or osteochondromas) that are often accompanied by skeletal deformities and short stature (2). In a small percentage of cases exostoses have exhibited malignant transformation resulting in an osteosarcoma or chondrosarcoma (3). Five members of this family have been cloned to date: EXT1, EXT2, EXTL1, EXTL2, and EXTL3. EXT1 and EXT2 are bifunctional enzymes with both beta-1,4-glucuronyltransferase and alpha-1,4-N-acetylglucosaminyltransferase activities that elongate HS chains with alternating beta-1,4-GlcA and alpha-1,4-GlcNAc residues on the non-reducing end (4). EXT1 is an active enzyme, but the hetero-dimer of EXT1 and EXT2 has increased stability and activity (1). The enzyme activity is measured using a phosphatase-coupled assay (5).

References

Busse, M. et al. (2007) J. Biol. Chem. 282:32802.
Raskind W.H. et al. (1998) Am. J. Hum. Genet. 62:346.
Hecht J.T. et al. (1997) Am. J. Hum. Genet. 60:80.
Kobayashi S. et al. (2000) Biochem. Biophys. Res. Commun. 268:860.
Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

Heterodimer of Exostosin 1 and Exostosin 2

Alternate Names

EXT1/EXT2 Heterodimer

UniProt

Q16394 (EXT1) & Q93063 (EXT2)

Additional Exostosin 1/2 Heterodimer Products

Product Documents for Recombinant Human Exostosin 1/2 Heterodimer Protein, CF

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human Exostosin 1/2 Heterodimer Protein, CF

For research use only

Citations
Reviews

Recombinant Human Exostosin 1/2 Heterodimer Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8567-GT

Key Product Details

Source

Accession #

Conjugate

Applications

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Product Specifications