Recombinant Human Exostosin 1 Protein, CF

Name: Recombinant Human Exostosin 1 Protein, CF
Brand: R&D Systems
SKU: 8379-GT

R&D Systems, part of Bio-Techne | Catalog # 8379-GT

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Product Datasheet / COA / SDS

New Version Available - 8567-GT

Discontinued Product

8379-GT has been discontinued. An alternative/replacement product is available: 8567-GT. View all Exostosin 1 products.

Key Product Details

Source:	CHO
Accession #:	Q16394
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human Exostosin 1 Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human Exostosin 1 Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human Exostosin 1 protein
Asp69-Leu746 with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Asp69 & Leu71

Predicted Molecular Mass

79 kDa

SDS-PAGE

79 kDa

Activity

Measured by its ability to transfer GlcNAc and GlcA from donor substrates to heparan sulfate. The specific activity is >300 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8379-GT

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: Exostosin 1

The exostosin (EXT) family of glycosyltransferases are involved in heparan sulfate (HS) and heparin biosynthesis (1). Mutations of these enzymes are the causes of hereditary multiple exostoses, which are characterized by formation of numerous cartilage-capped, benign bone tumors (osteocartilaginous exostoses or osteochondromas) that are often accompanied by skeletal deformities and short stature (2). In a small percentage of cases exostoses have exhibited malignant transformation resulting in an osteosarcoma or chondrosarcoma (3). Five members of this family have been cloned to date: EXT1, EXT2, EXTL1, EXTL2, and EXTL3. EXT1 and EXT2 are bifunctional enzymes with both beta -1,4-glucuronyltransferase and alpha -1,4-N-acetylglucosaminyltransferase activities that add alternating beta -1,4-GlcA and alpha -1,4-GlcNAc residues on the non-reducing end of HS chain (4). EXT1 is an active enzyme, but the hetero-dimer of EXT1 and EXT2 is more stable and has higher activity (1). The enzyme activity of recombinant human EXT1 is measured using a phosphatase-coupled assay (5).

Item	Value
References	Busse, M. et al. (2007) J. Biol. Chem. 282:32802. Wuyts, W. et al. (1998) Am. J. Hum. Genet. 62:346. Hecht J.T. et al. (1997) Am. J. Hum. Genet. 60:80. Kobayashi S. et al. (2000) Biochem. Biophys. Res. Commun. 268:860. Wu, Z.L. et al. (2011) Glycobiology 21:727.
Long Name	Exostosin Glycosyltransferase 1
Entrez Gene IDs	2131 (Human); 14042 (Mouse); 299907 (Rat)
Alternate Names	EC 2.4.1.224, EC 2.4.1.225, EXT, EXT1, Exostosin 1, Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan4-alpha-N-acetylglucosaminyltransferase, LGCR, LGS, Langer-Giedion syndrome chromosome region, Multiple exostoses protein 1, N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase, Putative tumor suppressor protein EXT1, TRPS2, TTV, exostoses (multiple) 1, exostosin-1

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Recombinant Human Exostosin 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8379-GT

Key Product Details

Product Specifications for Recombinant Human Exostosin 1 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Exostosin 1

Customer Reviews for Recombinant Human Exostosin 1 Protein, CF

Product Documents for Recombinant Human Exostosin 1 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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