Recombinant Human FABP8/M-FABP Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9487-CL

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9487-CL-100
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

P02689

Conjugate

Unconjugated

View all FABP8/M-FABP Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human FABP8/M-FABP protein
Ser2-Val132, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2

Predicted Molecular Mass

16 kDa

SDS-PAGE

16 kDa, reducing conditons

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9487-CL
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate.
Reconstitution
Reconstitute at 1 mg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP8/M-FABP

Fatty acid binding protein-8 (FABP8; also named Peripheral myelin protein 2, M- (myelin) FABP, Myelin P2 Protein, MP2, or P2) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1, 8, 9). FABP-8 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126-140 amino acids (aa) in length (1, 2, 3). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. The FABP family members are subdivided based on organ or tissue type it was originally expressed or identified; liver- (L-FABP), intestine- (I-FABP), heart- (H-FABP), adipocyte- (A-FABP), epidermal- (E-FABP), ileal- (Il-FABP), brain- (B-FABP), myelin- (M-FABP) and testis-FABP (T-FABP) (1). Human M-FABP, the product of the PMP2 gene, is a 131 aa cytosolic protein that shows a flattened beta -barrel structure generated by a series of antiparallel beta -strands and two alpha ‑helices (4, 7, 10). One molecule of FABP-8 is capable of binding one long-chain fatty acid (1, 5, 6). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in "internalization" of the ligand (5, 6, 7). Human FABP-8 is 87%, 92% and 83% aa identical to mouse, bovine and horse FABP-8, respectively. It also shows 26% and 30% aa identity to human L-FABP and I‑FABP, respectively.

References

  1. Smathers, R & Petersen, D. (2011) Human Genomics 5:170.
  2. Storch, J. & Thumser, AE. (2000) Biochim Biophys Acta. 1486:28.
  3. Zimmerman, A.W. & Veerkamp, J.H. (2007) Protein Sci. 9:2042.
  4. Jones, TA. (1988) The EMBO Journal. 7:1 597.
  5. Majava,V. et al. (2010) PLoS One. 5:e10300.
  6. Ruskamo, S. et al. (2014) Acta Crystallogr D Biol Crystallogr. 70:165.
  7. Bernlohr, D. et al. (1997) Ann. Rev. of Nut. 17:277.
  8. Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
  9. Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
  10. Suzuki, M. et al. (1982) J Neurochem. 39:1759.

Long Name

Fatty Acid-Binding Protein 8

Alternate Names

M-FABP, MFABP, MP2, PMP2

Entrez Gene IDs

5375 (Human); 18857 (Mouse); 688790 (Rat)

Gene Symbol

PMP2

UniProt

P02689 (Human)

Additional FABP8/M-FABP Products

Product Documents for Recombinant Human FABP8/M-FABP Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human FABP8/M-FABP Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.