Recombinant Human FAM20B Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8427-FM

R&D Systems, part of Bio-Techne
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8427-FM-050
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Key Product Details

Source

CHO

Accession #

O75063

Conjugate

Unconjugated

Applications

Bioactivity, Enzyme Activity

View all FAM20B Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human FAM20B protein
Asn27-Leu409, with an N-terminal HA tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

44 kDa

SDS-PAGE

44-53 kDa, reducing conditions

Activity

Measured by its ability to enhance survival of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.8-4 μg/mL.

Measured by its ability to phosphorylate D-xylose.
The specific activity is >15 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8427-FM
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 500 μg/mL in 25 mM Tris-HCl, pH7.5.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAM20B

FAM20B is a member of the FAM20 protein family that has three members in mammals (FAM20A, FAM20B, FAM20C) (1). Human FAM20B shares 97% amino acid sequence identity with mouse and rat FAM20B. FAM20B localizes to the Golgi apparatus where it phosphorylates the xylose residue in the glycosaminoglycan (GAG)-protein linkage region of proteoglycans, which leads to enhanced GAG biosynthesis (2). Accordingly, chondroitin sulfate and heparan sulfate production is increased when FAM20B is over-expressed and reduced following FAM20B knockdown (2). FAM20B knockout mice display embryonic lethality at day E13.5, suggesting that FAM20B has an important role during development (3). Furthermore, in zebrafish, FAM20B mutations result in reduced cartilage matrix production and skeletal defects (4). The enzymatic activity of recombinant human FAM20B is measured using a phosphatase-coupled method (5).

References

  1. Nalbant, D. et al. (2005) BMC Genomics 6:11.
  2. Koike, T. et al. (2009) Biochem. J. 421:157.
  3. Vogel, P. et al. (2012) Vet. Pathol. 49:998.
  4. Eames, B.F. et al. (2011) PLoS Genet. 7:e1002246.
  5. Wu, Z.L. (2011) PLoS One 6:e23172.

Long Name

Family with Sequence Similarity 20, Member B

Alternate Names

Glycosaminoglycan Xylosylkinase, gxk1, XYLK, Xylose Kinase

Entrez Gene IDs

9917 (Human); 215015 (Mouse); 304885 (Rat)

Gene Symbol

FAM20B

UniProt

O75063

Additional FAM20B Products

Product Documents for Recombinant Human FAM20B Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human FAM20B Protein, CF

For research use only

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