Recombinant Human FAM20C Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9265-FM

R&D Systems, part of Bio-Techne
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9265-FM-050
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Key Product Details

Source

CHO

Accession #

Q8IXL6

Conjugate

Unconjugated

Applications

Enzyme Activity

View all FAM20C Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human FAM20C protein
Val63-Arg584
with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val63 (pro-mature) and Asp93 (mature)

Predicted Molecular Mass

60 kDa

SDS-PAGE

70-82 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Universal Kinase Activity Kit (Catalog # EA004).
The specific activity is >20 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9265-FM
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: FAM20C

Fam20C is a protein kinase dedicated to the phosphorylation of extracellular proteins, including caseins, and is localizes within the Golgi apparatus and also is secreted (1, 2). It phosphorylates the caseins as well as several other secreted proteins such as AMELX, AMTN, ENAM, SPP1 and the small integrin-binding ligand N-linked glycoproteins SIBLINGs implicated in biomineralization (3). Consequently, mutations in Fam20C cause an osteosclerotic bone dysplasia in humans known as Raine syndrome (4) and hypophosphatemic rickets (5). The discovery of the function of Fam20C has solved a 130-year-old mystery of the identity of the kinases that phosphorylate caseins and has shed light on several human disorders of biomineralization (6). In addition to its role in biomineralization, Fam20C also plays a role in lipid homeostasis, wound healing and cell migration and adhesion (1).

References

  1. Tagliabracci, V. S. et al. (2013) cell 161:1619.
  2. Tagliabracci, V. S. et al. (2012) Science 336:6085.
  3. Cui, j. et al. (2015) Elife 4:e06120
  4. Fradin M, et al. (2011) Clin. Genet. 80:177.
  5. Wang X, et al. (2012) PLoS Genet. 8(5):e1002708.
  6. Tagliabracci VS, et al. (2013) Trends Biochem. Sci. 38:121.

Long Name

Family with Sequence Similarity 20, Member C

Alternate Names

DMP4, GEF-CK, RNS

Entrez Gene IDs

56975 (Human); 80752 (Mouse); 304334 (Rat)

Gene Symbol

FAM20C

UniProt

Q8IXL6

Additional FAM20C Products

Product Documents for Recombinant Human FAM20C Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human FAM20C Protein, CF

For research use only

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