Recombinant Human Fc gamma RIIA/CD32a Avi His Protein, CF
R&D Systems, part of Bio-Techne | Catalog # AVI1330
Biotinylated Avi-tag His-tag
Key Product Details
Learn more about Avi-tag Biotinylated Proteins
Source
HEK293
Accession #
Structure / Form
Biotinylated via Avi-tag
Conjugate
Biotin
Applications
Bioactivity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human Fc gamma RIIA/CD32a protein
Human Fc gamma RIIA (Ala36-Ile218) Accession # AAA35827.1 |
Avi-tag | 6-His tag |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala36
Predicted Molecular Mass
24 kDa
SDS-PAGE
32-39 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Human IgG is immobilized at 5.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Fc gamma RIIA/CD32a Avi-tag His-tag Protein binds with an ED50 of 1.00-8.00 μg/mL.
When Human IgG is immobilized at 5.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Fc gamma RIIA/CD32a Avi-tag His-tag Protein binds with an ED50 of 1.00-8.00 μg/mL.
Scientific Data Images for Recombinant Human Fc gamma RIIA/CD32a Avi His Protein, CF
Biotinylated Recombinant Human Fc gamma RIIA/CD32a Avi-tag His-tag Protein Binding Activity.
When Human IgG is immobilized at 5.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Fc gamma RIIA/CD32a Avi-tag His-tag Protein (Catalog # AVI1330) binds with an ED50 of 1.00-8.00 μg/mL.Biotinylated Recombinant Human Fc gamma RIIA/CD32a Avi-tag His-tag Protein SDS-PAGE.
2 μg/lane of Biotinylated Recombinant Human Fc gamma RIIA/CD32a Avi-tag His-tag Protein (Catalog # AVI1330) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-39 kDa.Formulation, Preparation and Storage
AVI1330
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Fc gamma RIIA/CD32a
References
- van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
- Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
- Takai, T. (2002) Nature Rev. Immunol. 2:580.
- Chi, M. et al. (2002) J. Immunol. 168:1413.
- Zuniga, R. et al. (2003) Arthritis Rheum. 48:460.
Long Name
Fc gamma Receptor II A
Alternate Names
CD32a, FCGR2A, FcgRIIA, FCRIIA
Gene Symbol
FCGR2A
UniProt
Additional Fc gamma RIIA/CD32a Products
Product Documents for Recombinant Human Fc gamma RIIA/CD32a Avi His Protein, CF
Product Specific Notices for Recombinant Human Fc gamma RIIA/CD32a Avi His Protein, CF
For research use only
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