Recombinant Human FGF-12 Protein

R&D Systems, part of Bio-Techne | Catalog # 2246-FG

R&D Systems, part of Bio-Techne
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Carrier Free
2246-FG-025/CF
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With Carrier
2246-FG-025
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Key Product Details

Source

E. coli

Accession #

NP_004104

Conjugate

Unconjugated

Applications

Binding Activity

View all FGF-12 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human FGF-12 protein
Met1-Thr181

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

20.4 kDa

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human FGFR4 Fc Chimera (Catalog # 685-FR) is immobilized at 2 μg/mL, 100 μL/well, Recombinant FGF-12 binds with an ED50 of 3-18 μg/mL.

Scientific Data Images for Recombinant Human FGF-12 Protein

Recombinant Human FGF-12 Protein Binding Activity

Recombinant Human FGF-12 Protein Binding Activity

When Recombinant rhFGF R4/Fc Chimera (685-FR) is immobilized at 2 μg/mL (100 μL/well), it binds to Recombinant Human FGF-12 (Catalog # 2246-FG) with an ED50 of 3-18 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2246-FG
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2246-FG/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-12

Fibroblast growth factor 12 (FGF-12) is a member of the FGF superfamily of molecules which contains at least 22 members. The FGFs are characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure, and all apparently bind heparin (1, 2). FGF-11, -12, -13, -14, originally termed FGF homologous factors (FHF) -3, -1, -2, -4, respectively, form a subgroup within the FGF family (1, 3). Human FGF-12/FHF-1 is synthesized as a 243 aa protein. It lacks a typical signal sequence and is considered to be a cytoplasmic protein. It does, however, possess an N-terminal bipartite nuclear localization signal (NLS) at aa 11-18 and 28-38 (4-6). The 243 aa protein has at least one alternate splice form that is 181 aa in length. This is termed FGF-12B. Alternate splicing deletes the N-terminal 66 aa in FGF-12 and replaces them with four aa in FGF-12B. This substitution removes the NLS from the short form. Studies on the short form (12B) show that it cannot bind any of the common FGF receptors. This is consistent with its cytoplasmic localization. It can, however, bind to IB2 (islet brain-2), a cellular kinase scaffold protein, and voltage-gated sodium channels, suggesting FGF-12B plays an important role in intracellular signaling and ion exchange (7). Mouse and human FGF-12B differ by only one amino acid.

References

  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Ornitz, D.M. and N. Itoh (2001) Genome Biol. 2:REVIEWS 3005.
  3. Green, P. et al. (1996) BioEssays 18:639.
  4. Smallwood, P. M. et al. (1996) Proc. Natl. Acad. Sci. 93:9850.
  5. Munoz-Sanjuan, I. et al. (2000) J. Biol. Chem 275:2589.
  6. Kok, L.D.S. et al. (1999) Biochem. Biophys. Res. Commun. 255:717.
  7. Olsen, S. K. et al. (2003) J. Biol. Chem. 278:34226.

Long Name

Fibroblast Growth Factor 12

Alternate Names

FGF12, FHF1

Entrez Gene IDs

2257 (Human)

Gene Symbol

FGF12

UniProt

NP_004104

Additional FGF-12 Products

Product Documents for Recombinant Human FGF-12 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 2246-FG (with carrier)
Download SDS 2246-FG/CF (carrier free)

Product Specific Notices for Recombinant Human FGF-12 Protein

For research use only

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