Recombinant Human FGF-18 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8988-F18

R&D Systems, part of Bio-Techne
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8988-F18-050
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Key Product Details

Source

E. coli

Accession #

O76093

Conjugate

Unconjugated

Applications

Bioactivity

View all FGF-18 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human FGF-18 protein
Glu28-Ala207, with a N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

21 kDa

SDS-PAGE

19-24 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGF RIIIc.
The ED50 for this effect is 4-24 ng/mL

Scientific Data Images for Recombinant Human FGF-18 Protein, CF

Recombinant Human FGF-18 Protein Bioactivity

Recombinant Human FGF-18 Protein Bioactivity

Recombinant Human FGF-18 (Catalog # 8988-F18) induces cell proliferation of the Baf3 mouse pro-B cells transfected with human FGF RIIIc. The ED50 for this effect is 4-24 ng/mL.

Formulation, Preparation and Storage

8988-F18
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-18

Fibroblast Growth Factor 18 (FGF-18) is a 20 kDa protein that plays an important role in skeletal development and bone homeostasis (1). Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18 (2). It is expressed in embryonic somites and the neural fold (3), adult lung (2), cerebellar and hippocampal neurons (4), hair follicle root sheath cells (5), and osteogenic mesenchymal cells (6). FGF-18 binds to FGF R2c, FGF R3c (4, 7) as well as the Golgi protein GLG1 (8) and induces the proliferation of astrocytes and microglia, vascular endothelial cells, dermal fibroblasts, papilla cells, and keratinocytes (4, 5, 9). FGF-18 is required for normal skeletal development (6). It recruits osteoclasts and osteoblasts to the growth plate, promotes osteoclast formation and function, inhibits osteoblast differentiation, promotes skeletal vascularization, and induces chondrocyte hypertrophy and cartilage matrix formation (6, 7, 10, 11).

References

  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Ohbayashi, N. et al. (1998) J. Biol. Chem. 273:18161.
  3. Ohuchi, H. et al. (2000) Mech. Dev. 95:55.
  4. Hoshikawa, M. et al. (2002) Brain Res. Mol. Brain Res. 105:60.
  5. Kawano, M. et al. (2005) J. Invest. Dermatol. 124:877.
  6. Ohbayashi, N. et al. (2002) Genes Dev. 16:870.
  7. Davidson, D. et al. (2005) J. Biol. Chem. 280:20509.
  8. Miyaoka, Y. et al. (2010) Development 137:159.
  9. Hu, M.C. et al. (1998) Mol. Cell. Biol. 18:6063.
  10. Liu, Z. et al. (2007) Dev. Biol. 302:80.
  11. Shimoaka, T. et al. (2002) J. Biol. Chem. 277:7493.

Long Name

Fibroblast Growth Factor 18

Alternate Names

FGF18

Entrez Gene IDs

8817 (Human)

Gene Symbol

FGF18

UniProt

O76093

Additional FGF-18 Products

Product Documents for Recombinant Human FGF-18 Protein, CF

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Product Specific Notices for Recombinant Human FGF-18 Protein, CF

For research use only

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