Recombinant Human FGFR1 Kinase Domain Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8657-FR

R&D Systems, part of Bio-Techne
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8657-FR-020
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P11362

Conjugate

Unconjugated

Applications

Enzyme Activity

View all FGFR1 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human FGF R1 protein
Met399-Arg822 with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.

Predicted Molecular Mass

49 kDa

SDS-PAGE

45-58 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >12 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8657-FR
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: FGFR1

Fibroblast growth factor receptors (FGF Rs) are single-pass transmembrane proteins with extracellular fibroblast growth factor (FGF) binding domains and cytoplasmic tyrosine-protein kinase domains (1). FGF Rs play essential roles in the regulation of embryonic development, cell proliferation, differentiation and migration (2). Upon FGF and heparan sulfate binding, the FGF Rs form homodimers and the kinase domains are activated (3, 4). The activated kinases then phosphorylate downstream effector molecules such as PLC-g1, FRS2, Gab1, and SHB, leading to the activation of several signaling cascades (5). For example, activation of PLC-g1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate (6). Phosphorylation of FRS2 triggers recruitment of GRB2, Gab1, PI 3-Kinase p85, and SOS1, activation of Ras, ERK2, and ERK1, and activation of the MAP kinase and AKT1 signaling pathways (7). In this construct, the kinase domain of human FGF R1 is expressed as a constitutively active enzyme (8) and the activity was assayed using a phosphatase-coupled method (9).

References

  1. Johnson, D.E. et al. (1990) Mol. Cell. Biol. 10:4728.
  2. Turner, N and Grose, R. (2010) Nat. Rev. Cancer 10:116.
  3. Pantoliano, M.W. et al (1994) Biochemistry 33:10229.
  4. Ornitz, D.M. et al. (996) J. Biol. Chem. 271:15292.
  5. Eswarakumar, V.P. et al. (2005) Cytokine Growth Factor Rev. 16:139.
  6. Peters, K.G. et al. (1992) Nature 358:678.
  7. Mohammadi, M. et al. (1996) Mol. Cell. Biol. 16:977.
  8. Mohammadi, M et al (1997) Science 276:955.
  9. Wu, Z.L. (2011) PloS ONE 6:e23172.

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human); 14182 (Mouse)

Gene Symbol

FGFR1

UniProt

P11362

Additional FGFR1 Products

Product Documents for Recombinant Human FGFR1 Kinase Domain Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human FGFR1 Kinase Domain Protein, CF

For research use only

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