Recombinant Human Fibromodulin/FMOD Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9840-FM

R&D Systems, part of Bio-Techne
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9840-FM-050
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Key Product Details

Source

NS0

Accession #

Q06828

Conjugate

Unconjugated

Applications

Bioactivity

View all Fibromodulin/FMOD Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Fibromodulin/FMOD protein
Asp75-Ile376, with an N-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

36 kDa

SDS-PAGE

41-55 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Bovine Collagen I is coated at 10 µg/mL (100 μL/well), Recombinant Human Fibromodulin/FMOD (Catalog # 9840-FM) binds with an ED50 of 0.250-2.25 µg/mL.

Scientific Data Images for Recombinant Human Fibromodulin/FMOD Protein, CF

Recombinant Human Fibromodulin/FMOD Protein Binding Activity

Recombinant Human Fibromodulin/FMOD Protein Binding Activity

When Bovine Collagen I is coated at 10 µg/mL (100 μL/well), Recombinant Human Fibromodulin/FMOD Protein (Catalog # 9840-FM) binds with an ED50 of 0.250-2.25 µg/mL.

Recombinant Human Fibromodulin/FMOD Protein SDS-PAGE.

2 μg/lane of Recombinant Human Fibromodulin/FMOD Protein (Catalog # 9840-FM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-55 kDa.

Formulation, Preparation and Storage

9840-FM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibromodulin/FMOD

Fibromodulin (FMOD) is a secreted, 60-70 kDa class II member of the small leucine-rich proteoglycan (SLRP) family of matrix proteins. It is expressed by a variety of cell types, including keratinocytes, fibroblasts and chrondrocytes, and is known to bind to type I and XII collagen, plus TGF-beta (1, 2). FMOD is posited to displace lumican binding to collagen, generating 3D collagen fibrils from simple lumican-induced collagen dimers (3). Mature human FMOD is 358 amino acids (aa) in length (aa 19-376). It contains a sulfated N-terminal complement C1q binding domain (aa 19-98) and 12 Leu-rich repeats (LRRs) (aa 98-376), with the LRR5-7 and LRR11 responsible for collagen type I binding (2 - 4). Fibromodulin contains varying amounts of keratan sulfate, and undergoes significant tyrosine sulfation (5). Over aa 76-376, human Fibromodulin shares 96% aa identity with mouse and rat Fibromodulin. Recent study has shown that FMOD can induce glioma cell migration and invasion by promoting actin cytoskeleton remodeling (6).

References

  1. Velez-Delvalle, C. et al. (2008) Biochem. Biophys. Res. Commun. 371:420.
  2. Kalamajski, S. et al. (2007) J. Biol. Chem. 282:26740.
  3. Kalamajski, S. et al. (2009) J. Biol. Chem. 284:534.
  4. Sjoberg, A. et al. (2005) J. Biol. Chem. 280:32301.
  5. Onnerfjord, P. et al. (2004) J. Biol. Chem. 279:26.
  6. Mondal, B. et al. (2017) Oncogene 36:71.

Alternate Names

FM, FMOD, SLRR2E

Entrez Gene IDs

2331 (Human); 14264 (Mouse); 64507 (Rat)

Gene Symbol

FMOD

UniProt

Q06828

Additional Fibromodulin/FMOD Products

Product Documents for Recombinant Human Fibromodulin/FMOD Protein, CF

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Product Specific Notices for Recombinant Human Fibromodulin/FMOD Protein, CF

For research use only

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