Recombinant Human Follistatin-like 5/FSTL5 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 9066-FN
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Follistatin-like 5/FSTL5 protein
Arg21-Ala847, with a C-terminal 6-His tag
Arg21-Ala847, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg21
Predicted Molecular Mass
94 kDa
SDS-PAGE
86-102 kDa, reducing conditions
Activity
Measured by its ability to inhibit Hep3B human hepatocellular carcinoma cell survival.
The ED50 for this effect is 3-12 μg/mL.
The ED50 for this effect is 3-12 μg/mL.
Scientific Data Images for Recombinant Human Follistatin-like 5/FSTL5 Protein, CF
Recombinant Human Follistatin-like 5/FSTL5 Protein Bioactivity
Recombinant Human Follistatin-like 5/FSTL5 (Catalog # 9066-FN) inhibits Hep3B human hepatocellular carcinoma cell survival. The ED50 for this effect is 3-12 μg/mL.Formulation, Preparation and Storage
9066-FN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 500 μg/mL in PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Follistatin-like 5/FSTL5
two EF-hand domains, and two Ig-like domains. Mature human FSTL5 shares 91% amino acid sequence identity with mouse and rat FSTL5. Alternative splicing generates an isoform with a 10 residue deletion following the second Ig-like domain. FSTL5 is expressed in the cartilage and tendon of developing digits and the shafts of long bones (2). In the brain it is expressed in the olfactory bulb, CA3 area of the hippocampus, and granule cell layer of the cerebellum (3). FSTL5 inhibits the proliferation and promotes the apoptosis of hepatocellular carcinoma cells (4). Its expression in medulloblastoma and down-regulation in hepatocellular carcinoma are associated with poor prognosis (4, 5).
References
- Bradshaw, A.D. (2012) Int. J. Biochem. Cell Biol. 44:480.
- Lorda-Diez, C.I. et al. (2013) PLoS One 8:e60423.
- Masuda, T. et al. (2014) Congenit. Anom. (Kyoto) 54:63.
- Zhang, D. et al. (2015) Int. J. Clin. Exp. Pathol. 8:3386.
- Remke, M. et al. (2011) J. Clin. Oncol. 29:3852.
Alternate Names
Follistatin like 5, FSL5, FSTL5
Gene Symbol
FSTL5
UniProt
Additional Follistatin-like 5/FSTL5 Products
Product Documents for Recombinant Human Follistatin-like 5/FSTL5 Protein, CF
Product Specific Notices for Recombinant Human Follistatin-like 5/FSTL5 Protein, CF
For research use only
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