Recombinant Human Frizzled-5 Fc Chimera (CHO-expressed), CF

R&D Systems, part of Bio-Techne | Catalog # 1617-FZC

R&D Systems, part of Bio-Techne
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1617-FZC-050
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Key Product Details

Source

CHO

Accession #

NP_003459

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Frizzled-5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Frizzled-5 protein
Human Frizzled-5
(Ala27-Trp238)
Accession # NP_003459		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27 and Ser28

Predicted Molecular Mass

50.1 kDa (monomer)

SDS-PAGE

60-68 kDa, reducing conditons

Activity

Measured by its binding ability in a functional ELISA.
In a 100 µL reaction mixture containing biotinylated recombinant mouse Wnt-5a at 100 ng/mL and Recombinant Human Frizzled‑5 Fc Chimera dilutions, the concentration of Recombinant Human Frizzled‑5 Fc Chimera that produces 50% of the maximal binding response is found to be approximately 5‑20 ng/mL.
Optimal concentrations should be determined by each laboratory for each application.

Formulation, Preparation and Storage

1617-FZC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-5

Frizzled-5 is an approximately 62 kDa 7-TM glycoprotein that is one of at least ten Frizzled family Wnt receptors (1, 2). Wnt engagement of Frizzled, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co‑receptors, stabilizes beta-catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzleds can also signal through non-canonical pathways independently of LRPs (1). Mature human Frizzled-5 consists of a 212 amino acid (aa) extracellular domain (ECD), a 283 aa 7-TM region, and a 64 aa cytoplasmic domain with a PDZ binding motif (2, 3). The ECD includes a cysteine-rich region (CRD) that binds Wnts and is highly conserved among Frizzled proteins (1, 2, 4). Within the N-terminal ECD, human Frizzled-5 shares 95% aa sequence identity with mouse and rat Frizzled-5. Frizzled-5 is expressed in embryonic tissues (telencephalon, pituitary, thalamus, hypothalamus, eye, liver, spleen, lung, and kidney), in the adult retina, colon, and pancreatic islets, some cancer cell lines, human embryonic stem cells, and in some monocyte and lymphocyte populations (2-8). Frizzled-5 functions as a receptor for Wnt-5a, Wnt-9b, Wnt-10b, Wnt-2b, and Wnt-7a (3, 5, 9, 10). It plays a role in the maintenance of yolk sac and placental vasculature (ishikawa) and in the regression of vitreous vasculature during eye development (5, 6, 11). It mediates the synaptogenic effect of Wnt-7a, contributes to the development of neuronal polarity, and is required for neuronal survival in the thalamus (3, 12, 13). In macrophages and monocytes, Frizzled-5 signaling induces the production of inflammatory cytokines (7, 8).

References

  1. Schulte, G. (2015) Eur. J. Pharmacol. 763:191.
  2. Saitoh T. et al. (2001) Int. J. Oncol. 19:105.
  3. Liu, C. et al. (2008) J. Neurosci. 28:5641.
  4. Katoh, Y. and M. Katoh (2007) Int. J. Oncol. 30:751.
  5. Ishikawa, T. et al. (2001) Development 128:25.
  6. Burns, C.J. et al. (2008) Dev. Dyn. 237:1614.
  7. Periera, C. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:504.
  8. Blumenthal, A. et al. (2006) Blood 108:965.
  9. He, X. et al. (1997) Science 275:1652.
  10. Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.
  11. Zhang, J. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5561.
  12. Sahores, M. et al. (2010) Development 137:2215.
  13. Slater, P.G. et al. (2013) PLoS One 8:e78892.

Alternate Names

Frizzled5, Frz-5, FZ-5, FZD5

Entrez Gene IDs

7855 (Human); 14367 (Mouse)

Gene Symbol

FZD5

UniProt

NP_003459

Additional Frizzled-5 Products

Product Documents for Recombinant Human Frizzled-5 Fc Chimera (CHO-expressed), CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Frizzled-5 Fc Chimera (CHO-expressed), CF

For research use only

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