Recombinant Human Fructosamine-3-kinase/FN3K Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9155-FU

R&D Systems, part of Bio-Techne
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9155-FU-050
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Key Product Details

Source

E. coli

Accession #

Q9H479

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Fructosamine-3-kinase/FN3K Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Fructosamine-3-kinase/FN3K protein
Glu2-Lys309, with N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

36 kDa

SDS-PAGE

37 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate 1-deoxy-1-morpholino-D-fructose.
The specific activity is >30 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9155-FU
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Fructosamine-3-kinase/FN3K

Nonenzymatic glycation (Maillard reaction) is a process in which glucose and other sugars react spontaneously with amine-containing molecules, such as proteins (1). These reactions proceed through many stages, starting with glucosylamines (Schiff bases), fructosamines (Amadori compounds) (2), and aminoaldoses (Heyns compounds) (3) and ultimately lead to the formation of irreversible end products, which include crosslinks, aromatic heterocycles, and oxidized compounds (4), that are designated collectively as advanced glycation end products (AGEs) (5). The concentrations of these products are elevated in diabetes, and important in the etiology of diabetes complications (6, 7). Fructosamine-3-phosphokinase (FN3K) is a kinase that phosphorylates fructoselysine and a wide variety of fructosamines, therefore removing these AGEs (8-10). Lost the protective enzymatic activity of FN3K is also associated with the oncology of colorectal cancer (11). FN3K is particularly active in brain, heart, kidney, and skeletal muscle and in erythrocytes (12,13). The kinase activity of recombinant human PFKFB3 was assayed using a phosphatase-coupled method (14).

References

  1. Monnier, V.M. (1989) Prog. Clin. Biol. Res. 304:1.
  2. Roper, H, et al. (1994). Carbohydr. Res. 262:257.
  3. Carson, J.F. et al. (1955). J. Am. Chem. So.c 77:1881.
  4. Reddy, S, et al. (1995) Biochemistry 34:10872.
  5. Bucala, R. and Cerami, A. (1992) Adv. Pharmacol. 23:1.
  6. Lyons, T.J. and Jenkins, A.J: (1997). Diabetes Rev. 5:365.
  7. Furusyo, N and Hayashi, J. (2013) Biochim. Biophys. Acta. 1830:5509.
  8. Lal, S, et al.(1993) J. Biol. Chem. 268:7763.
  9. Szwergold, B.S. et al. (2001) DIABETES 50:2139.
  10. Delpierre, G. et al. (2002) Biochem. J. 365: 801.
  11. Caruso, M.G. et al (2007) Oncology 73(1-2):72.
  12. Delplanque, J. et al. (2004) J. Biol.Chem. 279:46606.
  13. Petersen A, et al. (1992) Biochem J 284:363.
  14. Wu, Z.L. et al. (2011) PloS ONE 6(8):e23172.

Alternate Names

FN3K

Entrez Gene IDs

64122 (Human); 63828 (Mouse)

Gene Symbol

FN3K

UniProt

Q9H479

Additional Fructosamine-3-kinase/FN3K Products

Product Documents for Recombinant Human Fructosamine-3-kinase/FN3K Protein, CF

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Product Specific Notices for Recombinant Human Fructosamine-3-kinase/FN3K Protein, CF

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