Recombinant Human Fucosyltransferase 2/FUT2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7770-GT

R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

HEK293

Accession #

Q10981

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Fucosyltransferase 2/FUT2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Fucosyltransferase 2/FUT2 protein
Ile35-His343, with an C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile35

Predicted Molecular Mass

36 kDa

SDS-PAGE

40-50 kDa, reducing conditions

Activity

Measured by its ability to transfer fucose from GDP-fucose to lactose.
The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7770-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Fucosyltransferase 2/FUT2

Glycans are frequently fucosylated at terminal sites. Therefore, fucose is often part of a sugar epitope with important biological function. Well‑known fucose‑containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in leukocyte homing and the extravasation process, and thus are important for lymphocyte maturation and natural defense functions. Fucose‑containing glycans also play critical roles in cell signaling and development (1). More than 10 fucosyltransferases have been cloned, and most are Golgi‑resident type II membrane proteins (2). FUT3, FUT4, FUT5, FUT6, FUT7, and FUT9 are alpha1‑3 or  alpha1‑4 fucosyltransferases and are responsible for Lewis antigen generation (3, 4, 5). FUT8 is the only alpha1‑6 fucosyltransferase that adds a fucose to the chitobiose core of N‑glycans (6). FUT1 and FUT2 are galactoside alpha1‑2 fucosyltransferases that generate H‑antigen, the precursor for ABO blood‑group antigen synthesis. In particular, FUT2 involves in generating soluble ABO antigen in saliva; therefore it is also called secretor blood group alpha1‑2 fucosyltransferase (7). Absence of functional FUT2 is related to Crohn’s disease (8). The activity of this enzyme has been measured with a phosphatase‑coupled method (9).

References

  1. Jafar-Nejad, H. et al. (2010) Glycobiology 20:931.
  2. Becker, D.J. et al. (2003) Glycobiology 13:41R.
  3. Blander, J. M. et al. (1999) J. Immunol. 163:3746.
  4. Natsuka, S. et al. (1994) J. Biol. Chem. 269:16789.
  5. Sasaki, K. et al. (1994) J. Biol. Chem. 269:14730.
  6. Lee, S.H. et al. (2006) J. Biochem. 139:391.
  7. Kelly, R.J. et al. (1995) J. Biol. Chem. 270:4640.
  8. McGovern, D.P. et al. (2010) Hum. Mol. Genet. 19:3468.
  9. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Alternate Names

B12QTL1, FUT2, Se2, SEC2, SEJ

Entrez Gene IDs

2524 (Human); 14344 (Mouse); 58924 (Rat)

Gene Symbol

FUT2

UniProt

Q10981 (Human)

Additional Fucosyltransferase 2/FUT2 Products

Product Documents for Recombinant Human Fucosyltransferase 2/FUT2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Fucosyltransferase 2/FUT2 Protein, CF

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