Recombinant Human Fucosyltransferase 8/FUT8 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5768-GT

R&D Systems, part of Bio-Techne
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5768-GT-020
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

CHO

Accession #

Q9BYC5

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Fucosyltransferase 8/FUT8 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Fucosyltransferase 8/FUT8 protein
Asp32-Lys575, with an N-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

64 kDa

SDS-PAGE

58-62 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze the donor substrate GDP-fucose.
The specific activity is >0.75 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5768-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Fucosyltransferase 8/FUT8

N-glycans, O-glycans and glycolipids are frequently fucosylated at terminal sites. Therefore, fucose is often part of a sugar epitope with an important biological function. Well-known fucose-containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in leukocyte homing and extravasation and thus are important for lymphocyte maturation and natural defense functions. Fucose-containing glycans also play critical roles in cell signaling and development (1). More than 10 fucosyltransferases have been cloned and all of them are Golgi-resident type II membrane proteins (2). FUT1 and FUT2 are alpha1‑2 fucosyltransferases and are responsible for ABO blood-group antigen synthesis. FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are alpha1-3 or alpha1‑4 fucosyltransferases and are responsible for Lewis antigen generation (3, 4, 5). FUT8 is the only alpha1‑6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans (6). The alpha1,6‑fucosylation of N-glycan in human IgG1 was reported to suppress antibody-dependent cellular cytotoxicity (7, 8). Disruption of the FUT8 gene induced severe growth retardation, emphysema, and death during postnatal development in mice. The absence of alpha1,6-fucosylation on transforming growth factor-beta 1 (TGF-beta 1) receptors was found to be involved in the mouse phenotypes (9). The activity of this enzyme has been measured using a phosphatase‑coupled assay (10).

References

  1. Jafar-Nejad, H. et al. (2010) Glycobiology 20:931.
  2. Becker, D.J. et al. (2003) Glycobiology 13:41R.
  3. Blander, J. M. et al. (1999) J. Immunol. 163:3746.
  4. Natsuka, S. et al. (1994) J. Biol. Chem. 269:16789.
  5. Sasaki, K. et al. (1994) J. Biol. Chem. 269:14730.
  6. Lee, S.H. et al. (2006) J. Biochem. 139:391.
  7. Shields, R. L. et al. (2002) J. Biol. Chem. 277:26733.
  8. Shinkawa, T. et al. (2003) J. Biol. Chem. 278:3466.
  9. Wang, X. et al. (2005) Proc. Natl. Acad. Sci. U. S. A. 102:15791.
  10. Wu, Z.L., et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.

Alternate Names

alpha 1,6 Fucosyltransferase, alpha1-6FucT, FUT8

Entrez Gene IDs

2530 (Human); 53618 (Mouse); 432392 (Rat)

Gene Symbol

FUT8

UniProt

Q9BYC5

Additional Fucosyltransferase 8/FUT8 Products

Product Documents for Recombinant Human Fucosyltransferase 8/FUT8 Protein, CF

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Product Specific Notices for Recombinant Human Fucosyltransferase 8/FUT8 Protein, CF

For research use only

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