Recombinant Human Fucosyltransferase 9/FUT9 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9347-GT

FUT9 has been formulated so that it can be used in the cell surface glycoengineering of living cells, and does not affect cell viability or native phenotype apart from the intended impact on cell glycobiology.
R&D Systems, part of Bio-Techne
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9347-GT-020
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

CHO

Accession #

Q9Y231

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Fucosyltransferase 9/FUT9 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Fucosyltransferase 9/FUT9 protein
Thr33-Asn359

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr33

Predicted Molecular Mass

39 kDa

SDS-PAGE

40-50 kDa, reducing conditions

Activity

Measured by its ability to transfer fucose from GDP-fucose to N-Acetyllactosamine
The specific activity is >1,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9347-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Fucosyltransferase 9/FUT9

N-glycans, O-glycans and glycolipids are frequently fucosylated at terminal sites. Therefore, fucose is often part of a sugar epitope with important biological function. Well-known fucose-containing glycans include Lewis and ABO blood group antigens. Lewis epitopes are key elements involved in the leukocyte homing and extravasation process and thus are important for lymphocyte maturation and natural defense functions. Fucose-containing glycans also play critical roles in cell signaling and development (1). More than 10 fucosyltransferases have been cloned (2). FUT1 and FUT2 are alpha1-2 fucosyltransferases and are responsible for ABO blood-group antigen synthesis. FUT8 is an alpha1-6 fucosyltransferase that adds a fucose to the chitobiose core of N-glycans (3). FUT3, FUT4, FUT5, FUT6, FUT7 and FUT9 are alpha1-3 or alpha1-4 fucosyltransferases and are responsible for Lewis antigen generation. In particular, FUT9 synthesizes the Lewis X oligosaccharide (CD15) in the organ buds progressing in mesenchyma during embryogenesis and in mature granulocytes (4, 5). The activity of this enzyme has been measured with a phosphatase-coupled method (6).

References

  1. Jafar-Nejad, H. et al. (2010) Glycobiology 20:931.
  2. Becker, D.J. et al. (2003) Glycobiology 13:41R.
  3. Lee, S.H. et al. (2006) J. Biochem. 139:391.
  4. Kaneko M. et al. (1999) FEBS Lett. 452:237.
  5. Brito, C. et al. (2008) Biochimie. 90:1279.
  6. Wu, Z.L. et al. (2011) Glycobilogy 21:727.

Alternate Names

Alpha-(1,3)-Fucosyltransferase 9, Fucosyltransferase IX, FucT-IX, FUT9

Entrez Gene IDs

10690 (Human); 14348 (Mouse); 84597 (Rat)

Gene Symbol

FUT9

UniProt

Q9Y231

Additional Fucosyltransferase 9/FUT9 Products

Product Documents for Recombinant Human Fucosyltransferase 9/FUT9 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Fucosyltransferase 9/FUT9 Protein, CF

For research use only

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