Recombinant Human g-Glutamylcyclotransferase/CRF21, CF

R&D Systems, part of Bio-Techne | Catalog # 5086-CT

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
5086-CT-010
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

O75223

Conjugate

Unconjugated

Applications

Enzyme Activity

View all gamma-Glutamylcyclotransferase/CRF21 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human gamma-Glutamylcyclotransferase/CRF21 protein
Met1-Leu188

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

21 kDa

SDS-PAGE

20-22 kDa, reducing conditions

Activity

Measured by its ability to release L-alanine from gamma-glutamyl-L-alanine, with the detection of alanine by alanine dehydrogenase.
The specific activity is >40,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5086-CT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: gamma-Glutamylcyclotransferase/CRF21

Gamma-glutamylcyclotransferase (GGCT) is an essential enzyme in the gamma-glutamyl cycle that catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides. The enzyme plays an important role in glutathione homeostasis. GGCT is also known as cytochrome c-releasing factor 21(CRF-21). It induces the release of cytochrome c from mitochondria, leading to apoptosis (1). It is a 188 amino acid protein that consists of six beta-strands, five alpha-helices and four short 3­­10 helices (2). Up-regulation of the expression of GGCT has been found in various tumor tissues including lung, esophagus, stomach, bile duct, uterine cervix, colon and breast. (3, 4). Therefore, GGCT is a potential biomarker for numerous types of cancers.

References

  1. Masuda Y. et al. (2006) Biochem. Biophys. Res. Commun. 346:454.

  2. Oakley A.J. et al. (2008) J. Biol. Chem. 283:22031.

  3. Gromov P. et al. (2010) J. Proteome Res. 9:3941.

  4. Amano T. et al. (2012) J. Histochem. Cytochem. 60:76.

Long Name

Cytochrome c Releasing Factor 21

Alternate Names

C7orf24, CRF21, gammaGlutamylcyclotransferase, GCTG, GGC, GGCT, LOC79017

Entrez Gene IDs

79017 (Human); 110175 (Mouse); 685702 (Rat)

Gene Symbol

GGCT

UniProt

O75223 (Human)

Additional gamma-Glutamylcyclotransferase/CRF21 Products

Product Documents for Recombinant Human g-Glutamylcyclotransferase/CRF21, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human g-Glutamylcyclotransferase/CRF21, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.