Recombinant Human GAL3ST2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7719-ST

R&D Systems, part of Bio-Techne
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7719-ST-005
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Key Product Details

Source

CHO

Accession #

Q9H3Q3

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Galactose-3-O-sulfotransferase 2/GAL3ST2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Galactose-3-O-sulfotransferase 2/GAL3ST2 protein
His32-Ala398 with an N-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

43 kDa

SDS-PAGE

55-65 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to alpha-Lactose.
The specific activity is >800 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7719-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Galactose-3-O-sulfotransferase 2/GAL3ST2

Galactose-3-O-sulfotransferase 2 (GAL3ST2) is a type II Golgi resident transmembrane protein. It transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-3 hydroxyl group of nonreducing beta-galactosyl residues present on various glycans, such as N-acetyllactosamine, lactose, lacto-N-tetraose (Lc4), lacto-N-neotetraose (nLc4), type 1 oligosaccharides (Gal beta 1-3GlcNAc-R), type 2 oligosaccharides (Gal beta1-4GlcNAc-R), and core 1 O-glycans (Gal beta1-3GalNAc alpha1-Ser/Thr) (1). Given its broad substrate specificity, GAL3ST2 is capable of radioisotope labeling of non-reducing terminal galactose on glycoproteins or glycolipids with 35S. In addition, it can be used to generate galactin-4 and galactin-8 specific ligands (2, 3). The protein is widely expressed in various tissues (1). The enzymatic activity of the recombinant human GAL3ST2 is measured using a phosphatase-coupled assay (4).

References

  1. Honke, K. et al. (2001) J. Biol. Chem. 276: 267.
  2. Ideo, H. et al. (2002) Glycobiology 12:199.
  3. Ideo, H. et al. (2011) J. Biol. Chem. 286:11346.
  4. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

Galactose-3-O-sulfotransferase 2, GP3ST

Entrez Gene IDs

64090 (Human); 381334 (Mouse); 685402 (Rat)

Gene Symbol

GAL3ST2

UniProt

Q9H3Q3 (Human)

Additional Galactose-3-O-sulfotransferase 2/GAL3ST2 Products

Product Documents for Recombinant Human GAL3ST2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human GAL3ST2 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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