Recombinant Human GASP-1/WFIKKN2 Protein
R&D Systems, part of Bio-Techne | Catalog # 2070-GS
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human GASP-1/WFIKKN2 protein
Leu35-His576, with a C-terminal 6-His tag
Leu35-His576, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu35
Predicted Molecular Mass
60.7 kDa
SDS-PAGE
75-80 kDa, reducing conditions
Activity
Measured by its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenous leukemia cells.
The ED50 for this effect is 0.03-0.15 µg/mL, in the presence of 40 ng/mL of rmGDF-8.
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is approximately 1.5 nM, as measured in a reaction mixture containing 1.0 nM trypsin,10 mM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.
The ED50 for this effect is 0.03-0.15 µg/mL, in the presence of 40 ng/mL of rmGDF-8.
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is approximately 1.5 nM, as measured in a reaction mixture containing 1.0 nM trypsin,10 mM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 2070-GS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 2070-GS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: GASP-1/WFIKKN2
Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that copurify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass (1). The GASP-1 cDNA encodes a 571 amino acid protein that contains a 29 amino acid signal sequence and is comprised of many conserved domains: WAP, Follistatin/Kazal, Immunoglobulin, two tandem Kunitz, and Netrin (1). Two related human proteins which contain the same domain structure are called WFIKKN (based on the presence and order of these conserved domains) and WFIKKNRP (WFIKKN-related protein) (2). Mouse GASP-1 is homologous to human WFIKKN2, also know as WFIKKNRP and mouse GASP-2 to human WFIKKN. Human GASP-1 is the same protein as WFIKKN2. WAP, Follistatin, Kazal and Netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP-2 is in the pancreas, liver, and thymus (4). GASP-1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (1). In addition, GASP-1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP-1 is 90% and 55% identical to mouse GASP-1 and human GASP-2, respectively.
References
- Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
- Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
- Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
- Trexler, M. et al. (2002) Biol. Chem. 383:223.
Long Name
Growth and Differentiation Factor-associated Serum Protein-1
Alternate Names
GASP1, WFIKKN2, WFIKKNRP
Gene Symbol
WFIKKN2
UniProt
Additional GASP-1/WFIKKN2 Products
Product Documents for Recombinant Human GASP-1/WFIKKN2 Protein
Product Specific Notices for Recombinant Human GASP-1/WFIKKN2 Protein
For research use only
Loading...
Loading...
Loading...