Recombinant Human GCNT1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 7248-GT
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Glucosaminyl (N-acetyl) Transferase 1/GCNT1 protein
Arg33-His428, with a C-terminal 6-His tag
Arg33-His428, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg33
Predicted Molecular Mass
47 kDa
SDS-PAGE
50-57 kDa, reducing conditions
Activity
Measured by its ability to transfer GlcNAc from UDP-GlcNAc to B1-3 galactosyl-N-acetyl galactosamine.
The specific activity is >350 pmol/min/μg, as measured under the described conditions.
The specific activity is >350 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
7248-GT
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Glucosaminyl (N-acetyl) Transferase 1/GCNT1
Mucin-type O-glycans are initiated with an O-GalNAc attachment to a serine or threonine in a polypeptide. The O-GalNAc residues are subsequently extended by various glycosyltransferases resulting in different types of O-glycans. Most O-glycans contain the core 1 structure, Gal beta1-3GalNAc. Glucosaminyl (N-acetyl) Transferase 1 (GCNT1) converts the core 1 O-glycan to core 2 O-glycan, Gal beta1-3(GlcNAc beta1-6)GalNAc, via the addition of a GlcNAc residue (1, 2). Various ligand carbohydrates can be formed from core 2 branched oligosaccharides. For example, sialyl Lex in mucin‑type glycoproteins of blood cells can be formed from core 2 branched oligosaccharides (3, 4). The expression of GCNT1 was found to be associated with the progression of various types of cancer (5, 6, 7). The enzymatic activity of the recombinant GCNT1 is measured using a phosphatase-coupled method (8).
References
- Bierhuizen, M.F. (1993) Genes & Development 7:468.
- Yeh, J.C. et al. (1999) J. Biol. Chem. 274:3215.
- Hemmerich, S. et al. (1995) J. Biol. Chem. 270:12035.
- Wilkins, P.P. et al. (1996) J. Biol. Chem. 270:18732.
- Shimodaira, K. et al. (1997) Cancer Res. 57: 5201.
- Hatakyama, S. et al. (2010) Int. J. Cancer 127:1052.
- St Hill, C.A. et al. (2009) BMC Cancer 9:79.
- Wu, Z.L. et al. (2011) Glycobiology 21:727.
Long Name
Glucosaminyl (N-acetyl) Transferase 1 Core 2
Alternate Names
C2GNT-L, C2GNT1, Core 2 GNT, G6NT, NACGT2
Gene Symbol
GCNT1
UniProt
Additional Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Products
Product Documents for Recombinant Human GCNT1 Protein, CF
Product Specific Notices for Recombinant Human GCNT1 Protein, CF
For research use only
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