Recombinant Human Glucosamine (N-acetyl)-6-Sulfatase, CF
R&D Systems, part of Bio-Techne | Catalog # 2484-SUC
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Glucosamine (N-acetyl)-6-Sulfatase/GNS protein
Val37-Leu552 & Thr44-Leu552, both with a C-terminal 10-His tag
Val37-Leu552 & Thr44-Leu552, both with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val37 & Thr44
Predicted Molecular Mass
59 & 60 kDa
SDS-PAGE
94 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
The specific activity is >250 pmol/min/μg, as measured under the described conditions.
The specific activity is >250 pmol/min/μg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 2484-SUC in the following applications:
Formulation, Preparation and Storage
2484-SUC
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Glucosamine (N-acetyl)-6-Sulfatase/GNS
A member of the sulfatase family, GNS is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and keratan sulfate (1, 2). It hydrolyzes the 6-sulfate group of the N-acetyl-D-glucosamine 6-sulfate units of the GAG. GNS deficiency results in mucopolysaccharidosis type IIID (MPS IIID or Sanfilippo D Syndrome), an inborn error leading to lysosomal accumulation of heparan sulfate. MPS IIID has profound mental deterioration, hyperactivity, and relatively mild somatic manifestations. The deduced amino acid sequence of human GNS consists of a signal peptide (residues 1-36) and a mature chain (residues 37-552) that may be further processed into N-terminal and C-terminal fragments (3). rhGNS corresponds to the single chain and has sulfatase activity described in Activity Assay Protocol.
References
- Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
- Neufeld, E.F. and J. Muenzer (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) pp. 3421, New York, McGraw-Hill.
- Robertson, D.A. et al. (1992) Biochem. J. 288:539.
Alternate Names
Glucosamine6Sulfatase, GNS
Entrez Gene IDs
2799 (Human)
Gene Symbol
GNS
UniProt
Additional Glucosamine (N-acetyl)-6-Sulfatase/GNS Products
Product Documents for Recombinant Human Glucosamine (N-acetyl)-6-Sulfatase, CF
Product Specific Notices for Recombinant Human Glucosamine (N-acetyl)-6-Sulfatase, CF
For research use only
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