Recombinant Human Glutaminase His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10115-GL

R&D Systems, part of Bio-Techne
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10115-GL-020
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Key Product Details

Source

E. coli

Accession #

O94925-1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Glutaminase Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Glutaminase protein
Ser17-Leu669
with N-terminal Met and 6-His tag

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

73 kDa

SDS-PAGE

59-74 kDa, under reducing conditions

Activity

Measured by its ability to hydrolyze glutamine to glutamate.
The specific activity is >12000 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human Glutaminase His-tag Protein, CF

Recombinant Human Glutaminase His-tag Protein Enzyme Activity

Recombinant Human Glutaminase His-tag Protein Enzyme Activity

Recombinant Human Glutaminase His-tag (Catalog # 10115-GL) is measured by its ability to hydrolyze glutamine to glutamate. The activity (orange) is higher than the competitor's Glutaminase (green).
Recombinant Human Glutaminase His-tag Protein SDS-PAGE

Recombinant Human Glutaminase His-tag Protein SDS-PAGE

1 μg/lane of Recombinant Human Glutaminase His-tag (Catalog # 10115-GL) and 1 μg/lane of competitor Human Glutaminase was resolved with 4-20% SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining.

Formulation, Preparation and Storage

10115-GL
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP, Glycerol and CHAPS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Glutaminase

Glutaminase (GLS) catalyzes the synthesis of glutamate from glutamine. There are two mammalian isozymes of glutaminase: kidney-type encoded by the GLS gene, and liver-type encoded by the GLS2 gene. The two isozymes are known to have different localization, functionality, and immunological and molecular characteristics (1, 2). The GLS gene expresses two splice variants with a conserved catalytic domain (3): kidney-type glutaminase (KGA) and a C-terminal truncated variant known as glutaminase C. Both splice variants form active tetramers and are activated by phosphate (4). The kidney glutaminase isoform, GLS, catalyzes the first reaction in the primary pathway for renal catabolism of glutamine to glutamate. It contains a mitochondrial signaling peptide, a nuclear receptor box, a catalytic glutaminase domain, three ankyrin repeats and a KEN box (5).  GLS promotes respiration and ATP generation in the mitochondria by providing an alternative input to the citric acid cycle. GLS is upregulated in response to oncogenes (5-7) in several cancers (8, 9). Cancer cells with altered metabolism have been shown to depend on GLS activity and upregulated glutamine consumption (10) for growth in acute myeloid leukemina (11), breast cancer (12), colorectal cancer (13), kidney cancer (14), lung cancer (15), melanoma (16) and pancreatic cancer (17). Based on its role in cancer, GLS is a pharmaceutical target for cancer therapy (10, 12, 13, 18).

References

  1. Kovacevic, Z. et al. (1983) Physiol. Rev. 63:547.
  2. Marquez, J. et al. (2006) Neurochem. Int. 48:465.
  3. Ramachandran, S. et al. (2016) Oncotarget. 7:57943.
  4. Huang, Q. et al. (2018) J. Biol. Chem. 293:3535.
  5. Thangavelu, K. et al. (2012) Proc. Natl. Acad. Sci. 109:7705.
  6. Wise, D. et al. (2008) Proc. Natl. Adac. Sci. 105:18782.
  7. Gao, P. et al. (2009) Nature. 458:762.
  8. Cline, M. S. et al. (2013) Sci. Rep. 3:2652.
  9. Cancer Genome Atlas Research Network, et al. (2013) Nat. Genet. 45:1113.
  10. Katt, W. P. et al. (2017) Future Med. Chem. 9:223.
  11. Willems, L. et al. (2013) Blood 122:3521.
  12. Gross, M. I. et al. (2014) Mol. Cancer Ther. 13:890.
  13. Huang, F. et al. (2014) Int. J. Clin. Exp. Pathol. 7:1093.
  14. Shroff, E. H. et al. (2015) Proc. Natl. Acad. Sci. 112:6539.
  15. van den Heuvel, A. P. et al. (2012) Cancer Biol. Ther. 13:1185.
  16. Hernandez-Davies, J. E. et al. (2015) J. Trans. Med. 13:1.
  17. Son, J. et al. (2013) Nature. 496:101.
  18. Zimmermann, S.C. et al. (2018) J. Med. Chem. [Epub ahead of print].

Long Name

Glutaminase Kidney Isoform, Mitochondrial

Alternate Names

GLS, K-Glutaminase, L-Glutamine Amidohydrolase

Entrez Gene IDs

2744 (Human); 14660 (Mouse); 24398 (Rat)

Gene Symbol

GLS

UniProt

O94925-1

Additional Glutaminase Products

Product Documents for Recombinant Human Glutaminase His-tag Protein, CF

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