Recombinant Human Growth Hormone 2 Protein

R&D Systems, part of Bio-Techne | Catalog # 7668-GH

R&D Systems, part of Bio-Techne
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Carrier Free
7668-GH-025/CF
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With Carrier
7668-GH-025
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Key Product Details

Source

E. coli

Accession #

P01242

Conjugate

Unconjugated

Applications

Bioactivity

View all Growth Hormone 2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Growth Hormone 2 protein
Phe27-Phe217, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

22.4 kDa

SDS-PAGE

20 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using Nb2-11 rat lymphoma cells. Gout, P.W. et al. (1980) Cancer Res. 40:2433.
The ED50 for this effect is 0.2-1.2 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7668-GH
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7668-GH/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 200 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone 2

Growth Hormone 2 (GH2), also known as Growth Hormone Variant (GH‑V) and Placental Growth Hormone (PGH), is a secreted 22 kDa glycoprotein in the Placental Lactogen/Prolactin peptide hormone family. GH2 shares considerable structural and functional similarity with the pituitary‑derived Growth Hormone (GH) (1‑3). Mouse and rat orthologs of GH2 have not been identified, but human GH2 is bioactive in mice as well as rats (4, 5). Alternative splicing of human GH2 generates a 20 kDa isoform with a deletion of amino acids 32‑46 and isoforms with substituted C‑terminal regions (3, 6). GH2 is secreted by placental syncytiotrophoblasts into the maternal circulation beginning early in gestation and rising throughout pregnancy, as the level of circulating GH concommitantly decreases (1, 3, 7). GH2 is also present at lower concentrations in the fetal circulation and amniotic fluid (8‑10). It is elevated in the amniotic fluid of Down syndrome pregnancies, and maternal and fetal circulating levels may be either elevated or depressed during pre‑eclampsia (10‑12). Like GH, GH2 exerts its activities through the Growth Hormone Receptor (GHR) (13). It promotes trophoblast invasion of the uterine wall and the secretion of maternal IGF‑I (14, 15). Maternal serum levels of GH2 positively correlate with IGF‑I during the third trimester and with fetal birthweight (7, 16). GH2 induces maternal insulin resistance and exerts both somitogenic and anti‑lipogenic activities (4, 5, 13). The 20 kDa isoform of GH2 also binds GHR and shows full anti‑lipogenic activity, reduced somitogenic activity, and reduced diabetogenic activity (5, 13).

References

  1. McIntyre, H.D. et al. (2009) Curr. Diabetes Rev. 5:185.
  2. Igout, A. et al. (1988) Arch. Int. Physiol. Biochim. 96:63.
  3. Cooke, N.E. et al. (1988) J. Biol. Chem. 263:9001.
  4. Barbour, L.A. et al. (2002) Am. J. Obstet. Gynecol. 186:512.
  5. Vickers, M.H. et al. (2009) Am. J. Physiol. Endocrinol. Metab. 297:E629.
  6. Boguszewski, C.L. et al. (1988) J. Clin. Endocrinol. Metab. 83:2878.
  7. Fuglsang, J. et al. (2003) J. Clin. Endocrinol. 88:4355.
  8. Higgins, M.F. et al. (2012) PLoS ONE 7:e29164.
  9. Mittal, P. et al. (2008) Growth Horm. IGF Res. 18:174.
  10. Mittal, P. et al. (2007) J. Matern. Fetal Neonatal Med. 20:651.
  11. Sifakis, S. et al. (2009) Growth Horm. IGF Res. 19:121.
  12. Mannik, J. et al. (2012) Mol. Cell. Endocrinol. 355:180.
  13. Solomon, G. et al. (2006) Growth Horm. IGF Res. 16:297.
  14. Lacroix, M.C. et al. (2005) Endocrinology 146:2434.
  15. Caufriez, A. et al. (1993) Am. J. Physiol. 265:E572.
  16. Mannik, J. et al. (2010) J. Clin. Endocrinol. Metab. 95:2433.

Alternate Names

GH2, GHL, GHV

Entrez Gene IDs

2689 (Human)

Gene Symbol

GH2

UniProt

P01242 (Human)

Additional Growth Hormone 2 Products

Product Documents for Recombinant Human Growth Hormone 2 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 7668-GH (with carrier)
Download SDS 7668-GH/CF (carrier free)

Product Specific Notices for Recombinant Human Growth Hormone 2 Protein

For research use only

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