Recombinant Human HAI-2A Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1106-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human HAI-2A protein
Met1-Lys197, with a C-terminal 10-His tag
Met1-Lys197, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala28
Predicted Molecular Mass
21 kDa
SDS-PAGE
27 kDa and 32 kDa, reducing conditions
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <4.6 nM, as measured under the described conditions.
The IC50 value is <4.6 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1106-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile, deionized water.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: HAI-2A
Two alternatively spliced forms of HAI-2 have been found in human tissues (1). HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. Encoded by the SPINT2 gene, HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain (1‑5). Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trysin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa. In comparison, HAI-2A is a weaker inhibitor of factor VIIa-tissue factor, factors IXa, Xa, and XIIa. Recombinant HAI-2A prolonged the clotting time in an activated partial thromboplastin time assay.
References
- Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.
- Kawaguchi, T. et al. (1997) J. Biol. Chem. 272:27558.
- Marlor. C.W. et al. (1997) J. Biol. Chem. 272:12202.
- Muller-Pillasch, F. et al. (1998) Biochim. Biophys. Acta 1395:88.
- Delaria, K.A. et al. (1997) J. Biol. Chem. 272:12209.
Long Name
HGF Activator Inhibitor Type 2A
Alternate Names
HAI2A, Placental Bikunin, SPINT2
Entrez Gene IDs
10653 (Human)
Gene Symbol
SPINT2
UniProt
Additional HAI-2A Products
Product Documents for Recombinant Human HAI-2A Protein, CF
Product Specific Notices for Recombinant Human HAI-2A Protein, CF
For research use only
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