Recombinant Human Hexokinase 2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8179-HK

R&D Systems, part of Bio-Techne
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8179-HK-020
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Key Product Details

Source

E. coli

Accession #

P52789

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Hexokinase 2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Hexokinase 2 protein
Phe11-Arg917, with N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

102 kDa

SDS-PAGE

100-105 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate glucose.
The specific activity is >1,000 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 8179-HK in the following applications:

Formulation, Preparation and Storage

8179-HK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT, Glucose and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Hexokinase 2

Hexokinases phosphorylate hexose to form hexose 6-phosphate, the first step in hexose metabolism (1). Phosphorylation of a hexose adds charge to molecule thereby making it difficult to transport out of a cell. The hexose is therefore retained for intracellular metabolic processes, such as glycolysis or glycogen synthesis. In most organisms, glucose is the most important substrate of hexokinases and glucose-6-phosphate is the most important product. There are four mammalian hexokinases (2). Hexokinase 1, 2 and 3 are referred to as high-affinity hexokinases because their Km for glucose is below 1 mM. Hexokinase 4 is specific for glucose and is also referred to as glucokinase (3). Hexokinase 2 (HK2), also known as muscle form hexokinase, localizes to the outer membrane of mitochondria and is present in adipose tissue, skeletal muscle, and heart (4). The amino acids corresponding to the mitochondrial binding domain (5) have been removed in the recombinant enzyme. Like HK1, HK2 contains two homologous halves that may have evolved from an ancestral hexokinase through gene duplication and tandem ligation (6). Unlike HK1, in HK2 both the C-terminal and N-terminal portions are catalytically active with the N-terminal half having higher activity than the C-terminal half (7). In HK2 both the N-terminal and C-terminal halves exhibit product inhibition. HK2 overexpression is required for tumor growth making HK2 an attractive oncotarget (4). The enzymatic activity of recombinant human HK2 is measured using a phosphatase-coupled method (8).

References

  1. Aleshin, A.E. et al. (1998) Structure 6:39-50
  2. Takeda, J. et al. (1993) J. Biol. Chem. 268:15200.
  3. Lange, A.J. et al. (1991) Biochem. J. 277: 159.
  4. Patra, K.C. et al. (2013) Cancer Cell. 24:213.
  5. Bianchi M et al. (1998) Mol Cell Biochem 189:185.
  6. Ureta, T. (1982) Comp Biochem Physiol. B. 71B:549.
  7. Ahn, K.J. et al. (2009) BMB Rep. 42:350.
  8. Wu, Z.L. (2011) PLoS One 6:e23172.

Alternate Names

HK2, HKII, HXK2

Entrez Gene IDs

3099 (Human); 15277 (Mouse); 25059 (Rat)

Gene Symbol

HK2

UniProt

P52789

Additional Hexokinase 2 Products

Product Documents for Recombinant Human Hexokinase 2 Protein, CF

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Product Specific Notices for Recombinant Human Hexokinase 2 Protein, CF

For research use only

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