Recombinant Human HGF Propeptide Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 7057-HG
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human HGF protein
Gln32-Ser728
Gln32-Ser728
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln32 predicted, sequencing might be blocked
Predicted Molecular Mass
79.7 kDa
SDS-PAGE
84 kDa, reducing conditions
Activity
>20 ng of Recombinant Human HGF Propeptide is cleaved to release active HGF when treated for 4 hours at 37 °C with 5 ng of kallikrein.
Formulation, Preparation and Storage
7057-HG
| Formulation | Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl. |
| Reconstitution |
Reconstitute at 100 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: HGF
disulfide‑linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human Pro‑HGF shares 90%‑93% aa sequence identity with bovine, canine, feline, mouse, and rat Pro-HGF. HGF binds heparan‑sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of Integrin alpha2 beta1 in epithelial cells by a selective increase in alpha2 gene transcription (11). This Integrin serves as a Collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of Nectin‑1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).
References
- Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
- Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
- Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
- Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
- Nakamura, T. et al. (1989) Nature 342:440.
- Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
- Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
- Corso, S. et al. (2005) Trends Mol. Med. 11:284.
- Maeshima, A. et al. (2000) Kid. Int. 58:1511.
- Montesano, R. et al. (1991) Cell 67:901.
- Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
- Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
- Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
- Mineo, R. et al. (1994) Endocrinology 145:4355.
- Urbanek, K. et al. (2005) Circ. Res. 97:663.
Long Name
Hepatocyte Growth Factor
Alternate Names
DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF
Entrez Gene IDs
Gene Symbol
HGF
UniProt
Additional HGF Products
Product Documents for Recombinant Human HGF Propeptide Protein, CF
Product Specific Notices for Recombinant Human HGF Propeptide Protein, CF
For research use only
Loading...
Loading...
Loading...