Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL3/RBX1 Complex protein
Met1 - Ala768 with a N-terminal 10-His tag (CUL3); Met1 - His108 (RBX1)

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

92 kDa (CUL3), 12 kDa (RBX1)

Activity

Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.

Formulation, Preparation and Storage

E3-435

Formulation	Supplied as a solution in HEPES, NaCl, Glycerol and DTT.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Background: CUL3/RBX1 Complex

Cullin-3 (CUL3) is a core component of multiple BCR (BTB-CUL3-RBX) E3 Ubiquitin ligase complexes that mediate the ubiquitination of several classes of signaling and structural proteins. In the BCR complex, CUL3 serves as a scaffold that organizes one or more BTB (BR-C, Ttk and Bab, also known as a POZ domain) substrate recognition subunits with the RBX subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme. Substrate specificity of a BCR ligase is determined by the BTB domain protein(s) associated with the ligase, though BTB-independent CUL3 ligase activity has been reported. In vivo, the E3 ubiquitin ligase of the BCR complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.

References

Baek, K., et al. (2020) Nature 578:461.
Choo Y.Y and T. Hagen (2012) PLos One 7:e41350.
Davidge, B., et al. (2019) J. Cell Sci. 132:jcs233049.
Duda, D.M., et al. (2012) Mol. Cell 47:371.
Stogios, P.J. et al. (2005) Genome Biol. 6:R82.

Long Name

Cullin 3/Ring Box Protein 1

UniProt

Q13618.2 (CUL3); P62877.1 (RBX1)

Additional CUL3/RBX1 Complex Products

Product Documents for Recombinant Human His10-CUL3/RBX1 Complex Protein, CF

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human His10-CUL3/RBX1 Complex Protein, CF

For research use only

Citations
Reviews

Recombinant Human His10-CUL3/RBX1 Complex Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E3-435

Key Product Details

Source

Accession #

Conjugate

Applications

View all CUL3/RBX1 Complex Proteins and Enzymes »

Product Specifications