Recombinant Human His6- (DYKDDDDK)-p53 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # SP-452

Soon to be discontinued.
R&D Systems, part of Bio-Techne
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SP-452-020
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Key Product Details

Source

E. coli

Accession #

NP_000537.3

Conjugate

Unconjugated

View all p53 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human p53 protein
Glu2 - Asp393 with N-terminal 6-His and (DYKDDDDK) tags

Purity

>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

46 kDa

Formulation, Preparation and Storage

SP-452
Formulation Supplied as a solution in HEPES, NaCl, Glycerol and ZnCl2.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: p53

p53 is well known for its key role as a tumor suppressor protein.  It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73 (1,2). Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation (3). p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis, including  cell cycle regulatory proteins and members the Bcl-2 family (3). Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination (3-5). A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation (6,7).

References

  1. Arrowsmith, C.H. et al. (1999) Cell Death Differ. 6:1169.
  2. Dötsch, V. et al. (2010) Cold Spring Harb. Perspect. Biol. 2:a004887.
  3. Freed-Pastor, W.A. & C. Prives (2012) Genes Dev. 26:1268.
  4. Feng, Z. et al. (2011) J. Mol. Cell Biol. 3:44.
  5. Gu, B. & W.-G. Zhu (2012) Int. J. Biol. Sci. 8:672.
  6. Momand, J. et al. (1992) Cell 69:1237.
  7. Haupt, Y. et al. (1997) Nature 387:296.

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Gene Symbol

TP53

UniProt

NP_000537.3

Additional p53 Products

Product Documents for Recombinant Human His6- (DYKDDDDK)-p53 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human His6- (DYKDDDDK)-p53 Protein, CF

For research use only

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