Recombinant Human His6-NEDD4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E3-290

R&D Systems, part of Bio-Techne
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E3-290-050
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Key Product Details

Source

T. ni (baculovirus)

Accession #

P46934.4

Conjugate

Unconjugated

Applications

Bioactivity

View all NEDD4 Proteins and Enzymes »

Product Specifications

Source

Trichoplusia ni, T. ni (baculovirus)-derived human NEDD4 protein
Leu570 - Asp1319 with a N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

88 kDa

Activity

Recombinant Human NEDD 4 is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1)  enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.

Formulation, Preparation and Storage

E3-290
Formulation Supplied as a solution in HEPES, NaCl, DTT and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: NEDD4

NEDD4 (Neural precursor cell expressed developmentally down-regulated protein 4) is a Ubiquitin E3 ligase and the prototypical member of an extended family of HECT domain ligases.  Human family members include NEDD4, NEDD4-2, ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDDL1 and NEDDL2.  In addition to the HECT domain, NEDD4 family members also contain a C2 domain and one or more WW domains.  With a predicted molecular weight of 150 kDa, NEDD4 is expressed in most human tissues where it localizes primarily to the perinuclear and other membrane regions.  Evidence suggests that NEDD4 down-regulates multiple membrane proteins (including FGFR1, IGFR1, and multiple EGFR family members) in a Ubiquitin-dependent internalization and lysosomal degradation process.  NEDD4 also plays a role in facilitating the sequestration of alpha-synuclein to cellular endosomes.  

References

  1. Harvey, K.F. and S. Kumar (1999) Trends Cell Biol. 9:166.
  2. Maspero, E., et al. (2013) Nat. Struct. Mol. Biol. 20:696.
  3. Scheffner, M. and S. Kumar (2014) Biochim. Biophys. Acta. 1843:61.
  4. Sugeno, N., et al. (2014) J. Biol. Chem. 289:18137.
  5. Yang, B. and S. Kumar (2010) Cell Death Differ. 17:68.

Long Name

Neural Precursor Cell Expressed, Developmentally Down-regulated Protein 4

Alternate Names

RPF1

Entrez Gene IDs

4734 (Human); 17999 (Mouse); 25489 (Rat)

Gene Symbol

NEDD4

UniProt

P46934.4

Additional NEDD4 Products

Product Documents for Recombinant Human His6-NEDD4 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-NEDD4 Protein, CF

For research use only

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