Recombinant Human His6-UBE2F Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E2-679
Will be discontinued when existing inventory is gone.
Key Product Details
Product Specifications
Source
E. coli-derived human UBE2F protein
Met1 - Arg185 with a N-terminal 6-His tag
Met1 - Arg185 with a N-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
23 kDa
Activity
Recombinant Human His6-UBE2F is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2F concentration of 0.1-1 μM.
Formulation, Preparation and Storage
E2-679
| Formulation | Supplied as a solution in HEPES, NaCl, TCEP and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: UBE2F
Ubiquitin-conjugating Enzyme E2F (UBE2F) is a NEDD8-conjugating enzyme (E2) (1). It is a 185 amino acid (aa) protein with a predicted molecular weight of 21 kDa. The human protein exhibits approximately 96% aa sequence identity with its mouse and rat orthologs. UBE2F is involved the covalent attachment of NEDD8 to target proteins in a process termed neddylation. For example, UBE2F appears to participate in the neddyylation of Cullin 5 (1). Members of the Cullin family (Cullin 1-7) are scaffold proteins that can incorporate into multimolecular complexes termed Cullin RING Ubiquitin Ligases (CRLs) (2). CRLs make up the largest subset of Ubiquitin Ligases (E3), and their activity is promoted by neddylation of associated Cullin family proteins (3).
References
- Huang, D.T. et al. (2009) Mol. Cell 33:483.
- Sarikas, A. et al. (2011) Genome Biol. 12:220.
- Duda, D.M. et al. (2011) Curr. Opin. Struct. Biol. 21:257.
Long Name
Ubiquitin-conjugating Enzyme E2F
Alternate Names
NCE2
Gene Symbol
UBE2F
UniProt
Additional UBE2F Products
Product Documents for Recombinant Human His6-UBE2F Protein, CF
Product Specific Notices for Recombinant Human His6-UBE2F Protein, CF
For research use only
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