Recombinant Human His6-USP1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E-564
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1 protein
Met1 - Leu785 (Gly670Ala & Gly671Ala) with a C-terminal 6-His tag
Met1 - Leu785 (Gly670Ala & Gly671Ala) with a C-terminal 6-His tag
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
89 kDa
Activity
Recombinant Human USP1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1 concentration of 0.01-0.5 μM.
Formulation, Preparation and Storage
E-564
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: USP1
USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains.
References
- Cohn, M.A., et al. (2007) Mol. Cell 28:786.
- Huang, T.T., et al. (2006) Nat. Cell Biol. 8:339.
- Nijman, S.M.B., et al. (2005) Mol. Cell 17:331.
- Williams, S.A, et al. (2011) Cell 146:918.
Long Name
Ubiquitin Specific Protease 1
Alternate Names
Deubiquitinating Enzyme 1, Ubiquitin Carboxyl-Terminal Hydrolase 1, Ubiquitin Thioesterase 1, UBP
Gene Symbol
USP1
UniProt
Additional USP1 Products
Product Documents for Recombinant Human His6-USP1 Protein, CF
Product Specific Notices for Recombinant Human His6-USP1 Protein, CF
For research use only
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