Recombinant Human His6-USP15 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E-594
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP15 protein
Met1 - Asn981 with a C-terminal 6-His tag
Met1 - Asn981 with a C-terminal 6-His tag
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
113 kDa
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP15 concentration of 5-20 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555).
Formulation, Preparation and Storage
E-594
| Formulation | Supplied as a solution in HEPES, NaCl, TCEP and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: USP15
Ubiquitin carboxyl-terminal hydrolase 15 (USP15) is a specialized cysteine protease with a predicted molecular weight of 112 kDa. USP15 is a member of the peptidase C19 family and the human protein shares 98% amino acid sequence identity with its mouse ortholog. USP15 is a regulator of the TGF beta signaling pathway, where it may promote signaling strength by deubiquitination of SMAD proteins and/or the TGF betaR1. Other reported targets of USP15 include histone H2B, NF kappaBI alpha, MDM2 and human papillomavirus type 16 E6 protein.
References
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Eichhorn P.J., et al. (2012) Nat. Med. 18: 429.
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Long L., et al. (2014) J. Biol. Chem. 289: 8916.
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Voss R.M., et al. (2009) J. Virol. 83: 8885.
- Zou Q., et al. (2014) Nat. Immunol. 15: 562.
Long Name
Ubiquitin Specific Protease 15
Alternate Names
Gcap18, KIAA0529, Ubp109, UNPH-2, UNPH4
Gene Symbol
USP15
UniProt
Additional USP15 Products
Product Documents for Recombinant Human His6-USP15 Protein, CF
Product Specific Notices for Recombinant Human His6-USP15 Protein, CF
For research use only
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