Recombinant Human Histone Deacetylase 8 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4359-DA
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Histone Deacetylase 8/HDAC8 protein
Met1-Val377, with an N-terminal Met and 7-His tag
Met1-Val377, with an N-terminal Met and 7-His tag
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Met predicted
Predicted Molecular Mass
43 kDa
SDS-PAGE
48 kDa, reducing conditions
Activity
Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC (Catalog # ES016) in a coupled assay.
The specific activity is >1.5 pmol/min/µg, as measured under the described conditions.
The specific activity is >1.5 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4359-DA
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Histone Deacetylase 8/HDAC8
Human Histone Deacetylase 8 (HDAC8) is a member of the class I Histone Deacetylases (HDACs) (1, 2). HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells (3). HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer (4). Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation (5, 6). Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor trichostatin A (1).
References
- Hu, E. et al. (2000) J. Biol. Chem. 275:15254.
- Annemieke, J et al. (2003) Biochem. J. 370:737.
- Gray, S. and T. Ekstrom (2001) Exp. Cell Res. 262:75.
- Yang, X. and S. Gregoire (2005) Mol. Cel. Biol. 25:2873.
- Waltregny, D. et al. (2004) Am. J. Pathol. 165:553.
- De Levak, L. et al. (2006) Am. J. Surg. Pathol. 30:319.
Alternate Names
HDAC8, HDACL1, RPD3
Gene Symbol
HDAC8
UniProt
Additional Histone Deacetylase 8/HDAC8 Products
Product Documents for Recombinant Human Histone Deacetylase 8 Protein, CF
Product Specific Notices for Recombinant Human Histone Deacetylase 8 Protein, CF
For research use only
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