Recombinant Human HS3ST1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5968-ST

R&D Systems, part of Bio-Techne
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5968-ST-020
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Key Product Details

Source

CHO

Accession #

O14792

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 protein
Arg21-His307, with an N-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

35 kDa

SDS-PAGE

38-50 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to heparan sulfate.
The specific activity is >30 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5968-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris-HCl and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1

Heparan sulfate is a highly sulfated polysaccharide that can be found on cell surface and within extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is detached from the protein core and is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at 2‑O position by heparan sulfate 2‑O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at N, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), heparan sulfate 3‑O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST1 is a rate-limiting enzyme for generating an antithrombin-binding pentasaccharide epitope on heparan sulfate and heparin (5, 6). Unlike other sulfotransferases that have signal-anchor domains and are type II membrane integral proteins in Golgi apparatus, HS3ST1 lacks a transmembrane domain and is likely to be an intraluminal enzyme (7, 8). The enzyme activity of the recombinant HS3ST1 is measured using a phosphatase-coupled assay (9).

References

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.

  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.

  3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.

  4. Xu, D. et al. (2005) Biochem. J. 386:451.
  5. Rosenberg, R.D. et al. (1997) J. Clin. Invest. 100:S67.

  6. Esko, J.D and Lindahl, U. (2001) J. Clin. Invest. 108:169.

  7. Shworak, N.W. et al. (1997) J. Biol. Chem. 272:28008.

  8. Liu, J. et al. (1999) J. Biol. Chem. 274:5158.

  9. Prather, B. et al. (2012) Anal.Biochem.423:86

Alternate Names

3OST1, h3-OST-1

Entrez Gene IDs

9957 (Human); 15476 (Mouse); 84406 (Rat)

Gene Symbol

HS3ST1

UniProt

O14792

Additional Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Products

Product Documents for Recombinant Human HS3ST1 Protein, CF

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Product Specific Notices for Recombinant Human HS3ST1 Protein, CF

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