Recombinant Human HSP47 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9166-SH

R&D Systems, part of Bio-Techne
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9166-SH-050
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Key Product Details

Source

CHO

Accession #

P50454

Conjugate

Unconjugated

Applications

Bioactivity

View all HSP47 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human HSP47 protein
Ala19-Asp412, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19

Predicted Molecular Mass

44 kDa

SDS-PAGE

47-51 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human HSP47, immobilized at 5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.   

Scientific Data Images for Recombinant Human HSP47 His-tag Protein, CF

Recombinant Human HSP47 His-tag Protein SDS-PAGE

Recombinant Human HSP47 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human HSP47 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at ~50 kDa under R conditions.

Formulation, Preparation and Storage

9166-SH
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: HSP47

Heat Shock Protein 47 (HSP47), also known as Serpin-H1, is a 47 kDa collagen-binding stress protein localized in the endoplasmic reticulum (ER) of collagen-secreting cells (1). It is encoded by the SERPINH1 gene, belongs to the serpin family, and contains the typical serpin fold but has no serine protease inhibitory activity (1). Human HSP47 shares 96% aa sequence identity with mouse HSP47. HSP47 is a chaperone that specifically binds pro-collagens at a neutral pH via Gly-Xaa-Arg repeats located on triple-helical procollagen to prevent aggregate formation (2). HSP47 is required for correct procollagen folding in the ER (1). Expression of HSP47 is directly correlated with expression of collagens in multiple types of cells and tissues. Gene disruption of HSP47 in mice causes embryonic lethality due to defects in collagen biosynthesis (1, 3) and HSP47-knockout cells show abnormal helix formation resulting in thin, branched fibrils (1, 4). HSP47 is associated with collagen-related disorders such as osteogenesis imperfecta (5) and fibrosis of the liver, lung, and other organs (6, 7). Its role in collagen regulation, as well as its ability to cause ER stress-mediated apoptosis in collagen-producing cells (6), make HSP47 a promising target for treatment in collagen-related diseases (7, 8). Additionally, HSP47 modulates the tumor microenvironment (9) and may serve as a predictive marker in patients with colorectal, renal, and laryngeal squamous cell carcinomas (10-12).

References

  1. Ito S. and Nagata K. (2017) Seminars in Cell & Developmental Biology. 62:142.
  2. Widmer, C. et al. (2012) Proc. Natl. Am. Soc. 109:13243.
  3. Nagai N. et al. (2000) J. Cell Biol., 150: 1499.
  4. Matsuoka Y. et al. (2004) Mol. Biol. Cell, 15: 4467.
  5. Christiansen, H. E. et al. (2010) Am. J. Hum. Genet. 86:389.
  6. Kawasaki, K. et al. (2015) J. Biol. Chem. 290:3639.
  7. Ito, S. et al. (2017) J. Biol. Chem. 292:20076.
  8. Otsuka, M. et al. (2017) Exp. Lung Res. 43:271.
  9. Jiang X. et al. ACS Chem Neurosci. (2017) 8:128.
  10. Mori K. et al. (2017) Int J Cancer. 140: 1425.
  11. Song, X. et al. (2017) Oncol. Rep. 38:2444.
  12. Qi, Y. et al. (2018) J. Cell Mol. Med. 22:1224.

Long Name

Heat Shock Protein 47

Alternate Names

AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1

Entrez Gene IDs

871 (Human); 12406 (Mouse); 29345 (Rat)

Gene Symbol

SERPINH1

UniProt

P50454

Additional HSP47 Products

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Product Specific Notices for Recombinant Human HSP47 His-tag Protein, CF

For research use only

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