Recombinant Human HTRA1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2916-SE

R&D Systems, part of Bio-Techne
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2916-SE-020
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Key Product Details

Source

E. coli

Accession #

Q92743

Conjugate

Unconjugated

Applications

Enzyme Activity

View all HTRA1/PRSS11 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human HTRA1/PRSS11 protein
Gly156-Pro480, with an N-terminal Met and C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

36 kDa

SDS-PAGE

37-40 kDa, reducing conditions

Activity

Measured by its ability to cleave beta-casein.
>95% of beta-Casein is cleaved by Recombinant Human HTRA1/PRSS11, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 2916-SE in the following applications:

Formulation, Preparation and Storage

2916-SE
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: HTRA1/PRSS11

HTRA1 is a member of the mammalian HTRA (high temperature requirement A) serine protease family. The E. coli HTRA homolog functions as a chaperone-protease and is essential for bacterial survival at temperatures above 42 °C (1). Among the four mammalian HTRA proteins, HTRA1, -3 and -4 are secreted while HTRA2 is localized in mitochondria. HTRA1 contains an N-terminal insulin-like growth factor binding protein (IGFBP) domain, a Kazal-type trypsin inhibitor motif, and C-terminal trypsin-like protease and PDZ domains (2). It is involved in several pathologies including Alzheimer’s disease (3), rheumatoid arthritis (4), osteoarthritis (5) and age‑related macular degeneration (6), although the mechanisms by which HTRA1 exerts its effects are not clear. HTRA1 also has properties of a tumor suppressor protein, which makes it a target for cancer therapy (7). In addition HTRA1 is known to regulate the TGF-beta signaling pathway and bone mineralization (8, 9). Recombinant human HTRA1 lacks the N-terminal IGFBP and Kazal-like domains, but retains the serine protease and PDZ domains.

References

  1. Jiang, J. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:11939.
  2. Murwantoko et al. (2004) Biochem. J. 381:895.
  3. Grau, S. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:6021.
  4. Grau, S. et al. (2006) J. Biol. Chem. 281:6124.
  5. Hu, S. et al. (1998) J. Biol. Chem. 273:34406.
  6. Dewan, A. et al. (2006) Science 314:989.
  7. Chien, J. et al. (2004) Oncogene 23:1636.
  8. Oka, C. et al. (2004) Development 131:1041.
  9. Hadfield, K.D. et al. (2008) J. Biol. Chem. 283:5928.

Long Name

High Temperature Requirement Protein-1

Alternate Names

IGFBP5-protease, PRSS11

Entrez Gene IDs

5654 (Human)

Gene Symbol

HTRA1

UniProt

Q92743

Additional HTRA1/PRSS11 Products

Product Documents for Recombinant Human HTRA1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human HTRA1 Protein, CF

For research use only

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