Recombinant Human HTRA2/Omi Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1458-HT
Key Product Details
Product Specifications
Source
E. coli-derived human HTRA2/Omi protein
Ala134-Glu458, with a C-terminal 6-His tag
Ala134-Glu458, with a C-terminal 6-His tag
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
N-terminal Sequence Analysis
Ala134
Predicted Molecular Mass
36 kDa
SDS-PAGE
38 kDa, reducing conditions
Activity
Measured by its ability to cleave beta-casein.
>95% cleavage of beta-casein, as measured under the described conditions.
>95% cleavage of beta-casein, as measured under the described conditions.
Formulation, Preparation and Storage
1458-HT
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: HTRA2/Omi
HtrA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HtrA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HtrA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (Catalog # http://www.rndsystems.com/product_results.aspx?k=786-XB">786-XB) inhibition of Caspase-7 (Catalog # http://www.rndsystems.com/product_results.aspx?k=823-C7">823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC50 values for this effect are typically between 0.2 and 1.5 μM. HtrA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site. The specificity of the protease is yet to be defined and no endogenous substrates are known to date.
References
- Suzuki, Y. et al. (2001) Mol. Cell. 8:613.
- van Loo, G. et al. (2002) Cell Death & Diff. 9:20.
- Hedge, R. et al. (2001) J. Biol. Chem. 277:432.
- Verhagen, A. et al. (2001) J. Biol. Chem. 277:445.
- Martins, L. et al. (2002) J. Biol. Chem. 277:439.
- Silke, J., and A. Verhagen (2002) Cell Death & Diff. 9:362.
- Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.
Long Name
High Temperature Requirement Protein-2
Alternate Names
Omi, PRSS25
Gene Symbol
HTRA2
UniProt
Additional HTRA2/Omi Products
Product Documents for Recombinant Human HTRA2/Omi Protein, CF
Product Specific Notices for Recombinant Human HTRA2/Omi Protein, CF
For research use only
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