Recombinant Human Hydroxyacid Oxidase-1/HAO-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6197-GO

R&D Systems, part of Bio-Techne
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6197-GO-010
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Key Product Details

Source

E. coli

Accession #

Q9UJM8

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Hydroxyacid Oxidase-1/HAO-1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Hydroxyacid Oxidase-1/HAO-1 protein
Leu2-Ile370, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

42 kDa

SDS-PAGE

40 kDa, reducing conditions

Activity

Measured by its ability to oxidize glyoxylate.
The specific activity is >200 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6197-GO
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Hydroxyacid Oxidase-1/HAO-1

Glycolate oxidase is a member of the superfamily of the alpha‑hydroxy acid oxidases (HAO), enzymes that are present in both plants and animals (1). It catalyzes the FMN-mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide (2, 3). The co‑factor, FMN, is tightly bound but not covalently linked to the protein. In humans and other vertebrates, HAOs are found primarily in the peroxisomes of liver, kidney, and pancreas. Three HAOs have been identified in humans (4). HAO-1 is most highly expressed in liver and pancreas and is most active on two-carbon substrates such as glycolate. HAO-2 is expressed in liver and kidney and has greater activity against long-chain alpha‑hydroxy acid substrates such as 2‑hydroxypalmitate. HAO-3 is expressed primarily in the pancreas. Recently, HAO has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones (5).

References

  1. Caroline, V. et al. (2007) Arch. Biochem. Biophys. 465:410.
  2. Murray, M.S. et al. (2008) Biochemistry, 47:2439.
  3. Pennati, A. and G. Gadda. (2009) J. Biol. Chem. 284:31214.
  4. Jones, J.M. et al. (2000) J. Biol. Chem. 275:12590.
  5. Monico, G.C. et al. (2002) Kidney Int. 62:392.

Alternate Names

GOX, HAO-1, HAO1, HAOX1, Hydroxyacid Oxidase1

Entrez Gene IDs

54363 (Human)

Gene Symbol

HAO1

UniProt

Q9UJM8 (Human)

Additional Hydroxyacid Oxidase-1/HAO-1 Products

Product Documents for Recombinant Human Hydroxyacid Oxidase-1/HAO-1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Hydroxyacid Oxidase-1/HAO-1 Protein, CF

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