Recombinant Human ICOS (aa 21-140) mFc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11423-CS

R&D Systems, part of Bio-Techne
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11423-CS-050
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Key Product Details

Source

CHO

Accession #

Q9Y6W8.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ICOS Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ICOS protein
Human ICOS
(Glu21-Lys140)
Accession # Q9Y6W8.1		 IEGRMD Mouse IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

41 kDa

SDS-PAGE

54-60 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human ICOS mFc Chimera (Catalog # 11423-CS) binds to Recombinant Human B7-H2 Fc Chimera Protein (Catalog # 165-B7) with a ED50 of 1.50-15.0 ng/mL.

Scientific Data Images for Recombinant Human ICOS (aa 21-140) mFc Chimera Protein, CF

Recombinant Human ICOS mFc Chimera Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human ICOS mFc Chimera Protein (Catalog # 11423-CS) binds to Recombinant Human B7-H2 Fc Chimera Protein (165-B7) with a ED50 of 1.50-15.0 ng/mL.

Recombinant Human ICOS mFc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human ICOS mFc Chimera Protein (Catalog # 11423-CS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-60 kDa and 108-120 kDa, respectively.

Formulation, Preparation and Storage

11423-CS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICOS

Inducible co-stimulator (ICOS), also called AILIM (activation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune costimulatory receptors (1). Other family members are CD28, CTLA-4 and PD-1 (2, 4). Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells (3). ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells (2, 4, 6). ICOS has been shown to have a role in the expansion of Th17 cells by regulating IL-21 production (8). B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand (4, 6). The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation (5, 7).

References

  1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  2. Coyle, A.J. et al. (2000) Immunity 13:95.
  3. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  5. Hutloff, A. et al. (1999) Nature 397:263.
  6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.
  8. Bauquet, AT. et al. (2009) Nat. Immunol. 10:167.

Long Name

Inducible Co-stimulator

Alternate Names

AILIM, CD278, CRP-1

Entrez Gene IDs

29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)

Gene Symbol

ICOS

UniProt

Q9Y6W8.1

Additional ICOS Products

Product Documents for Recombinant Human ICOS (aa 21-140) mFc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human ICOS (aa 21-140) mFc Chimera Protein, CF

For research use only

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