Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF
R&D Systems, part of Bio-Techne | Catalog # BT169
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Biotin
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ICOS protein
| Human ICOS (Glu21-Phe141) Accession # Q9Y6W8 |
IEGRMD | Human
IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu21
Predicted Molecular Mass
40 kDa (unlabeled)
SDS-PAGE
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED50 of 1-6 ng/mL.
When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED50 of 1-6 ng/mL.
Scientific Data Images for Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF
Recombinant Human ICOS Fc Chimera Biotinylated Protein Binding Activity
When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant ICOS Fc Chimera (Catalog # BT169) binds with an ED50 of 1-6 ng/mL.Formulation, Preparation and Storage
BT169
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution |
Reconstitute at 400 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ICOS
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune co-stimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
References
- Aicher, A. et al. (2000) J. Immunol. 164:4689.
- Coyle, A.J. et al. (2000) Immunity 13:95.
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
- Hutloff, A. et al. (1999) Nature 397:263.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
Inducible Co-stimulator
Alternate Names
AILIM, CD278, CRP-1
Gene Symbol
ICOS
UniProt
Additional ICOS Products
Product Documents for Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF
Product Specific Notices for Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF
For research use only
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