Recombinant Human IDO Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6030-AO

Indoleamine 2,3-dioxygenase
R&D Systems, part of Bio-Techne
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6030-AO-010
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Key Product Details

Source

E. coli

Accession #

P14902

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Indoleamine 2,3-dioxygenase/IDO Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Indoleamine 2,3-dioxygenase/IDO protein
M HHHHHH Human IDO
(Ala2-Gly403)
Accession # P14902
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

46 kDa

SDS-PAGE

42 kDa, reducing conditions

Activity

Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine.
The specific activity is >500 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 6030-AO in the following applications:

Formulation, Preparation and Storage

6030-AO
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Indoleamine 2,3-dioxygenase/IDO

Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N‑formyl‑kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN‑ gamma, IFN‑ alpha, IFN‑ beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3‑5).

References

  1. Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.

  2. Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.

  3. Xu, H. et al. (2008) Immunol. Lett. 121:1.

  4. Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.

  5. Curti, A. et al. (2009) Blood 113:2394.

Alternate Names

3dioxygenase, IDO, IDO1, INDO, Indoleamine 2

Entrez Gene IDs

3620 (Human); 15930 (Mouse)

Gene Symbol

IDO1

UniProt

P14902

Additional Indoleamine 2,3-dioxygenase/IDO Products

Product Documents for Recombinant Human IDO Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IDO Protein, CF

For research use only

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