Recombinant Human IFN-alpha-2A Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10984-IF

R&D Systems, part of Bio-Techne
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10984-IF-010
10984-IF-050
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Key Product Details

Source

HEK293

Accession #

P01563.1

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-alpha Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IFN-alpha protein
Cys24-Glu188

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-24 kDa, under reducing conditions.

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 fot this effect is 3.00-30.0 pg/mL.

Reviewed Applications

Read 1 review rated 4 using 10984-IF in the following applications:

Scientific Data Images for Recombinant Human IFN-alpha-2A Protein, CF

Recombinant Human IFN-alpha-2A Protein Bioactivity.

Recombinant Human IFNA-2A (Catalog # 10984-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 3.00-30.0 pg/mL

Recombinant Human IFN-alpha-2A Protein SDS-PAGE.

2 μg/lane of Recombinant Human IFN-alpha-2A Protein (Catalog # 10984-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-24 kDa.

Formulation, Preparation and Storage

10984-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80%amino acid (aa) sequence homology (2 - 4). There are several variants of human IFNA2, IFNA2a, IFNA2b and IFNA2c, which only differ by a few point mutations (5). The extracellular domain (ECD) of mature human IFNA2A, shares 59% aa sequence identity with mouse IFNA2. The type I IFNs bind to t e interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (6, 7). While individual IFN alpha subtypes are known to display unique efficacies to viral protection, nothing is known about functions unique to IFNA2 in human physiology. However, IFNA2 has become the representative type I IFN subtype used in basic research and numerous clinical applications (8). Since being first approved for treatment of hairy cell leukemia in 1986, IFNA2a has been approved for the treatment of several malignancies including AIDS-related Kaposi's sarcoma, chronic hepatitis C and chronic myelogenous leukemia (9).

References

  1. Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
  2. Goeddel, D.V. et al. (1980) Nature 287:411.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  5. Lee, N. et al. (1995) J. Interferon Cytokine Res. 15:341.
  6. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  7. James, C.M. et al. (2007) Vaccine. 25(10):1856.
  8. Paul, F. et al. (2015) Gene. 567:132.
  9. Kiladjian, J.J. et al. (2008) Leukemia 22:1990.

Long Name

Interferon alpha

Alternate Names

IFNalpha, LeIF D

Entrez Gene IDs

3440 (Human)

Gene Symbol

IFNA2

UniProt

P01563.1 (Human)

Additional IFN-alpha Products

Product Documents for Recombinant Human IFN-alpha-2A Protein, CF

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Product Specific Notices for Recombinant Human IFN-alpha-2A Protein, CF

For research use only

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