Recombinant Human IFN-alpha-4A Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10998-IF

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

P05014.2

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-alpha 4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IFN-alpha 4 protein
Cys24-Asp189

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19 kDa

SDS-PAGE

17-23 kDa, under reducing conditions.

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 for this effect is 1.00-20.0 pg/mL.

Scientific Data Images for Recombinant Human IFN-alpha-4A Protein, CF

Recombinant Human IFN-alpha-4A Protein Bioactivity.

Recombinant Human IFN-alpha-4A Protein (Catalog # 10998-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 1.00-20.0 pg/mL

Recombinant Human IFN-alpha-4A Protein SDS-PAGE.

2 μg/lane of Recombinant Human IFN-alpha-4A Protein (Catalog # 10998-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-23 kDa, under reducing conditions.

Formulation, Preparation and Storage

10998-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 4

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2-4). Structurally, type I IFNs belong to the class of five helical‑bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds (5). The mature human IFNA4 shares 57% aa sequence identity with mouse IFNA4. Two variants of human IFNA4 are known to exist, IFNA4a and IFNA4b, which only differ by residues at aa position 74 and 137 (6). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (7, 8). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA4 has been shown to be an intermediate inducer of IFN-stimulated genes and anti-viral protection (9). Mutations in IFNA4 can cause impaired function and lead to Crohn's disease susceptibility (10). IFNA4 also exhibits strong antiviral effects against SARS-CoV-2 (11).

References

  1. Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
  2. Goeddel, D.V. et al. (1980) Nature 287:411.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  5. Wittling, M.C. et al. (2021) Front Immunol. 11:605673.
  6. Hussain, M. et al. (1997) J. Interferon Cytokine Res. 17:559.
  7. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  8. James, C.M. et al. (2007) Vaccine. 25(10):1856.
  9. Moll, H.P. et al. (2011) Cytokine. 53:52.
  10. Xiao, C.X. et al. (2015) Sci Rep 5:10514.
  11. Schuhenn, J. et al. (2022) PNAS https://doi.org/10.1073/pnas.2111600119.

Long Name

Interferon alpha 4

Alternate Names

IFNA4, IFNalpha 4

Entrez Gene IDs

3441 (Human); 15967 (Mouse)

Gene Symbol

IFNA4

UniProt

P05014.2

Additional IFN-alpha 4 Products

Product Documents for Recombinant Human IFN-alpha-4A Protein, CF

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Product Specific Notices for Recombinant Human IFN-alpha-4A Protein, CF

For research use only

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