Recombinant Human IFN-alpha A (alpha 2a) Protein

R&D Systems, part of Bio-Techne | Catalog # 11100-1

R&D Systems, part of Bio-Techne
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11100-1
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Key Product Details

Source

E. coli

Accession #

V00549

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-alpha 2/IFNA2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IFN-alpha A protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

19.2 kDa

Activity

Interferon was titrated with the use of the cytopathic effect inhibition assay as described:

Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.

Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc.Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml.

The units are determined by use of a Human IFN Alpha A (Hu-IFN- alphaA [2a]) laboratory standard calibrated to the international reference standard for human interferon alpha A (Hu-IFN- alphaA [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].

Reviewed Applications

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Formulation, Preparation and Storage

11100-1
Formulation

Phosphate, buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Background: IFN-alpha 2/IFNA2

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name

Interferon alpha 2

Alternate Names

Ifa2, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2

Entrez Gene IDs

3440 (Human); 15965 (Mouse); 298213 (Rat)

Gene Symbol

IFNA2

UniProt

V00549

Additional IFN-alpha 2/IFNA2 Products

Product Documents for Recombinant Human IFN-alpha A (alpha 2a) Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IFN-alpha A (alpha 2a) Protein

For research use only

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