Recombinant Human IFN-gamma R2 Fc Chimera Avi Protein, CF
R&D Systems, part of Bio-Techne | Catalog # AVI11578
Key Product Details
Source
Structure / Form
Biotinylated via Avi-tag
Conjugate
Applications
Product Specifications
Source
Human IFNGR2 (Ala22-Gln247) Accession # AAA16955.1 |
GGIEGRMDGG | Human IgG1 (Pro100-Lys330) |
Avi-tag |
N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Biotinylated Recombinant Human IFN-gamma R2 Fc Chimera Avi-tag (Catalog # AVI11578) binds Recombinant Human IFN-gamma (Catalog # 285-IF) in the presence of Recombinant Human IFN-gamma R1/CD119 (Catalog # 673-IR/CF) with an ED50 of 0.0800-1.20 μg/mL.
Scientific Data Images for Recombinant Human IFN-gamma R2 Fc Chimera Avi Protein, CF
Biotinylated Recombinant Human IFN-gamma R2 Fc Chimera Avi-tag Protein Binding Activity.
Biotinylated Recombinant Human IFN-gamma R2 Fc Chimera Avi-tag (Catalog # AVI11578) binds Recombinant Human IFN-gamma (285-IF) in the presence of Recombinant Human IFN-gamma R1/CD119 (673-IR/CF) with an ED50 of 0.0800-1.20 μg/mL.Biotinylated Recombinant Human IFN-gamma R2 Fc Chimera Avi-tag Protein SDS-PAGE.
2 μg/lane of Biotinylated Recombinant Human IFN-gamma R2 Fc Chimera Avi-tag Protein (Catalog # AVI11578) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-74 kDa and 130-150 kDa, respectively.Formulation, Preparation and Storage
AVI11578
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IFN-gamma R2
IFN‑ gamma R2 (Interferon gamma receptor 2; also called IFN‑ gamma R beta IFN‑ gamma RII, or AF1) is a 60‑64 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecules (1). It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFN‑ gamma, the complex contains two each of IFN‑ gamma R1, R2 and Jak1 molecules (2). Binding of IFN‑ gamma to IFN‑ gamma R1 recruits Jak2 to IFN‑ gamma R2 and initiates phosphorylation, STAT1 binding, conformational changes, and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis (2, 3). Within the ECD, human IFN‑ gamma R2 shares 56% aa sequence identity with mouse IFN‑ gamma R2. IFN‑ gamma R1 and R2 must be from the same species for receptor complexes to be active, and human IFN‑ gamma is not active on the mouse IFN‑ gamma receptor complex (1, 2). IFN‑ gamma R1 is essential for ligand binding and is more constitutively expressed, while IFN‑ gamma R2 is essential for signaling, and its more limited expression controls cell response to IFN‑ gamma (2, 3). For example, mouse T cell IFN‑ gamma R2 is down‑regulated during differentiation to subtypes such as Th1 which produce IFN‑ gamma. (3, 4) This allows expansion of activated cells without growth arrest due to paracrine response to IFN‑ gamma. Following expansion, IFN‑ gamma R2 is re‑expressed to limit the immune reaction (5). IFN‑ gamma signaling mediates control of intracellular pathogens such as mycobacteria (3, 4, 6). In humans, deficiency of IFN‑ gamma R2 or other IFN‑ gamma pathway molecules causes the MSMD (mendelian susceptibility to mycobacterial diseases) syndrome (6‑8). Our Avi-tag Biotinylated human IFN‑ gamma R2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
References
- Hemmi, S. et al. (1994) Cell 76:803.
- Krause, C.D. et al. (2006) Cell Res. 16:55.
- Haring, J. S. et al. (2005) J. Immunol. 174:6791.
- Tau, G.Z. et al. (2000) J. Exp. Med. 192:977.
- Foulds, K.E. et al. (2008) J. Immunol. 180:842.
- Rosenzweig, S.D. et al. (2004) J. Immunol. 173:4000.
- Filipe-Santos. O. et al. (2006) Semin. Immunol. 18:347.
- Zhang, S-Y. et al. (2008) Immunol. Rev. 226:29.
Long Name
Alternate Names
Gene Symbol
Additional IFN-gamma R2 Products
Product Documents for Recombinant Human IFN-gamma R2 Fc Chimera Avi Protein, CF
Product Specific Notices for Recombinant Human IFN-gamma R2 Fc Chimera Avi Protein, CF
For research use only