Recombinant Human IGFBP-rp1/IGFBP-7 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10163-B7

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

Q16270

Conjugate

Unconjugated

Applications

Bioactivity

View all IGFBP-rp1/IGFBP-7 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IGFBP-rp1/IGFBP-7 protein
Ser27-Leu282

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

26 kDa

SDS-PAGE

29-34 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IGFBP-rp1/IGFBP-7 is used at 500 ng/mL (100 μL/well), the concentration of Recombinant Human CCL21/6Ckine (Catalog # 366-6C) that produces 50% optimal binding response is 2-12 ng/mL.

Scientific Data Images for Recombinant Human IGFBP-rp1/IGFBP-7 Protein, CF

Recombinant Human IGFBP-rp1/IGFBP-7 Protein Binding Activity

Recombinant Human IGFBP-rp1/IGFBP-7 Protein Binding Activity

When Recombinant Human IGFBP-rp1/IGFBP-7 (Catalog # 10163-B7) is used at 500 ng/mL, 100 µL/well, Recombinant Human CCL21/6Ckine (Catalog # 366-6C) binds with an ED50 of 2-12 ng/mL.
Recombinant Human IGFBP-rp1/IGFBP-7 Protein SDS-PAGE

Recombinant Human IGFBP-rp1/IGFBP-7 Protein SDS-PAGE

2 µg/lane of Recombinant Human IGFNP-rp1/IGFBP-7 (Catalog # 10163-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing major bands at 29-34 kDa and 25-30 kDa, respectively.

Formulation, Preparation and Storage

10163-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-rp1/IGFBP-7

IGFBP-rp1, also known as IGFBP-7, Mac25/Angiomodulin (AGM), tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide. Mature IGFBP-rp1 is a glycosylated protein with an N-terminal IGFBP domain, followed by a Kazal-type serine proteinase inhibitor domain and a C-terminal immunoglobulin-like C2-type domain. The similarity of IGFBP-rp1 with the IGFBPs is confined to the N-terminal IGFBP domain, which contains all 12 of the conserved cysteine residues found in IGFBP1 through 5. Human and mouse IGFBP-rP1 are highly homologous. Human and mouse IGFBP-rp1 share 94% aa sequence identity. IGFBP‑rp1 is expressed in many normal tissues and in cancer cells. It is abundantly expressed in high endothelial venules (HEVs) of blood vessels in the secondary lymphoid tissues. The expression of IGFBP-rp1 is up-regulated in senescing epithelial cells and by retinoic acid. IGFBP-rp1 binds IGF and insulin with very low affinity and has been shown to enhance the mitogenic actions of IGF and insulin. IGFBP-rp1 also has IGF/insulin-independent activities. It interacts with heparan sulfate proteoglycans, type IV collagen, and specific chemokines. IGFBP-rp1 supports weak cell adhesion, promotes cell spreading on type IV collagen, and stimulates the production of the potent vasodilator PGI2. It modulates tumor cell growth and has also been implicated in angiogenesis. IGFBP-rp1 is proteolytically cleaved between lysine 97 and alanine 98. Cleaved IGFBP-rp1 has enhanced cell attachment activity but can no longer bind IGF/insulin (1-3).

References

  1. Hwa, V. et al. (1999) Endocrinology Rev. 20:761.
  2. Nagakubo, D. et al. (2003) J. Immunol. 171:553.
  3. Ahmed, S. et al. (2003) Biochem. Biophys. Res. Commun. 310:612.

Long Name

Insulin-like Growth Factor Binding Protein Related Protein 1

Alternate Names

IGFBP-7, IGFBP7, IGFBPrp1, Mac25, PSF

Entrez Gene IDs

3490 (Human); 29817 (Mouse)

Gene Symbol

IGFBP7

UniProt

Q16270

Additional IGFBP-rp1/IGFBP-7 Products

Product Documents for Recombinant Human IGFBP-rp1/IGFBP-7 Protein, CF

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Product Specific Notices for Recombinant Human IGFBP-rp1/IGFBP-7 Protein, CF

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