Recombinant Human IgG1 Fc Protein, CF Best Seller
R&D Systems, part of Bio-Techne | Catalog # 110-HG
Analyzed by SEC-MALS
Key Product Details
Source
NS0
Accession #
Structure / Form
Dimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human IgG1 protein
| IEGRMD | Human IgG1 (Pro100-Lys330) Substitutions: Leu117Ala, Leu118Glu, Gly120Ala, Thr276Ala Accession # P01857.1 |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ile
Predicted Molecular Mass
26.6 kDa
SDS-PAGE
30-35 kDa, reducing conditions
Activity
Bioassay data are not available.
Reviewed Applications
Read 9 reviews rated 4.7 using 110-HG in the following applications:
Scientific Data Images for Recombinant Human IgG1 Fc Protein, CF
Recombinant Human IgG1 Fc Protein SEC-MALS.
Recombinant Human IgG1/Fc Protein (Catalog # 110-HG) has a molecular weight (MW) of 55.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a dimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Formulation, Preparation and Storage
110-HG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IgG1
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Additional IgG1 Products
Product Documents for Recombinant Human IgG1 Fc Protein, CF
Product Specific Notices for Recombinant Human IgG1 Fc Protein, CF
For research use only
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