Recombinant Human IL-13 R alpha2 Fc Alexa Fluor® 647 Protein

R&D Systems, part of Bio-Techne | Catalog # AFR7147

R&D Systems, part of Bio-Techne
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AFR7147-020
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Key Product Details

Learn more about FluorokinesTM Fluorescent-Labeled Proteins

Source

CHO

Accession #

NP_000631.1

Structure / Form

Disulfide-linked homodimer
Labeled with Alexa Fluor® 647
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm

Conjugate

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Bioactivity

View all IL-13 R alpha 2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-13 R alpha 2 protein
Human IL-13 R alpha 2
(Cys22-Leu342)
Accession # NP_000631.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys22

Predicted Molecular Mass

64 kDa (monomer)

SDS-PAGE

75-95 kDa, under reducing conditions.

Activity

Measured by flow cytometry for its ability to bind anti-human IL‑13 R alpha2 Antibody conjugated beads. The concentration of Recombinant Human IL‑13 R alpha2 Fc Chimera Alexa Fluor® 647 (Catalog # AFR7147) that produces 50% of the binding response is 1.50-15.0 ng/mL.

Scientific Data Images for Recombinant Human IL-13 R alpha2 Fc Alexa Fluor® 647 Protein

Flow cytometry analysis for Recombinant Human IL‑13 R alpha2 Fc Chimera Alexa Fluor® 647 staining on anti-human IL‑13 R alpha2 Antibody conjugated beads.

Streptavidin coated beads conjugated to biotinylated anti-human IL‑13 R alpha2 Polyclonal Antibody (BAF614) were stained with the indicated concentrations of Recombinant Human IL‑13 R alpha2 Fc Chimera Alexa Fluor® 647 (Catalog # AFR7147).

Formulation, Preparation and Storage

AFR7147
Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: IL-13 R alpha 2

Interleukin‑13 Receptor alpha 2 (IL‑13 R alpha2), also known as IL‑13 binding protein, and CD213a2, is a widely expressed 55 kDa cytokine receptor that plays an important role in the Th2‑polarized immune responses characteristic of a variety of pathologies, including parasitic infections and allergic asthma (1, 2). Mature human IL‑13 R alpha2 consists of a 317 amino acid (aa) extracellular domain with three fibronectin type‑III domains, a WSxWS motif, a 20 aa transmembrane segment, and a 17 aa cytoplasmic domain (3). Within the ECD, human IL‑13 R alpha2 shares 64% and 62% aa sequence identity with mouse and rat IL‑13 R alpha2, respectively. In both mouse and human, a 40 kDa‑50 kDa soluble form of IL‑13 R alpha2 can be generated by MMP‑8 mediated shedding in vitro (4).  Although this is assumed to occur in vivo in mouse, there is no evidence that shedding occurs in human (5‑7).  In mouse, alternative splicing also leads to sIL‑13 R alpha2, but again, this phenomenon apparently does not occur in human (6‑7).  Thus, the biological effects of human IL‑13 R alpha2 would appear to be mediated exclusively by membrane IL‑13 R alpha2 (7). The biological effects of IL‑13 and IL‑4 are closely related in part due to a shared receptor system. IL‑13 binds to IL‑13 R alpha1 which then forms a signaling complex with IL‑4 R alpha (8, 9). IL‑13 R alpha2 functions as a decoy receptor by binding and internalizing IL‑13 and preventing it from signaling through the IL‑13 R alpha1/IL‑4 R alpha complex (3, 10). IL‑13 R alpha2 can also block IL‑4 induced responses by inhibiting IL‑4 bound IL‑13 R alpha1/IL‑4 R alpha receptor complexes even though it does not itself bind IL‑4 (11, 12). Aside from its decoy function, IL‑13‑activated IL‑13 R alpha2 directly promotes the development of tissue fibrosis by inducing the transcription of TGF‑ beta (13). Presumably, any human soluble IL‑13 R alpha2, if it exists, will retain its ligand binding capability and attenuate responses to IL‑13 but not to IL‑4 (11, 14). The up‑regulation of transmembrane during Th2‑biased immune responses limits the extent of those responses (15‑17).

References

  1. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  2. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  3. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
  4. Chen, W. et al. (2008) J. Allergy Clin. Immunol. 122:625.
  5. O’Toole, M. et al. (2008) Clin. Exp. Allergy 38:594.
  6. Chen, W. et al. (2009) J. Immunol. 183:7870.
  7. Kasaian, M.T. et al. (2011) J. Immunol. 187:561.
  8. Andrews, A.-L. et al. (2006) J. Immunol. 176:7456.
  9. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
  10. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
  11. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  12. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
  13. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.
  14. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
  15. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  16. Morimoto, M. et al. (2009) J. Immunol. 183:1934.
  17. Zheng, T. et al. (2008) J. Immunol. 180:522.

Long Name

Interleukin 13 Receptor alpha 2

Alternate Names

CD213a2, IL-13Ra2, IL13R alpha 2, IL13RA2

Entrez Gene IDs

3598 (Human); 16165 (Mouse)

Gene Symbol

IL13RA2

UniProt

NP_000631.1 (Human)

Additional IL-13 R alpha 2 Products

Product Documents for Recombinant Human IL-13 R alpha2 Fc Alexa Fluor® 647 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human IL-13 R alpha2 Fc Alexa Fluor® 647 Protein

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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